Analytical sensibility and specificity of two RT-qPCR protocols for SARS-CoV-2 detection performed in an automated workflow
Gustavo Barcelos Barra, Ticiane Henriques Santa Rita, Pedro Goes Mesquita, Rafael Henriques Jacomo, Lidia Freire Abdalla Nery
doi: https://doi.org/10.1101/2020.03.07.20032326
Abstract
The World Health Organization declared that COVID-19 outbreak constituted a Public Health Emergency of International Concern and the development of reliable laboratory diagnosis of SARS-CoV-2 became mandatory to identify, isolate and provide optimized care for patients early. RT-qPCR testing of respiratory secretions is routinely used to detect causative viruses in acute respiratory infection. RT-qPCR in-house protocols to detect the SARS-CoV-2 have been described. Validations of these protocols are considered a key knowledge gap for COVID-19, especially if executed in a high throughput format.
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