Evaluation of simple nucleic acid extraction methods for the detection of SARS-CoV-2 in nasopharyngeal and saliva specimens during global shortage of extraction kits
Author links open overlay panelAllen Wing-HoChuaWan-MuiChanaJonathan DanielIpaCyril Chik-YanYipbJasper Fuk-WooChanabKwok-YungYuenabKelvin Kai-WangToab
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https://doi.org/10.1016/j.jcv.2020.104519
Abstract
Background
The severe shortage of nucleic acid extraction kits during the current COVID-19 pandemic represents a key limiting factor in testing capacity.
Objectives
This study compared the results of SARS-CoV-2 RT-PCR using different simple nucleic acid extraction methods on nasopharyngeal and saliva specimens.