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《CMI，6月23日，Large-scale implementation of pooled RNA extraction and RT-PCR for SARS-CoV-2 detection》

  • 来源专题：COVID-19科研动态监测
  • 编译者： zhangmin
  • 发布时间：2020-06-24

  • Large-scale implementation of pooled RNA extraction and RT-PCR for SARS-CoV-2 detection

    Author links open overlay panelRoniBen-Ami1#AgnesKlochendler1#MatanSeidel2#TalSido3OriGurel-Gurevich4MoranYassour56EranMeshorer7GilBenedek8IritFogel8EstherOiknine-Djian8AsafGertler8ZeevRotstein8BrunoLavi8YuvalDor1Dana G.Wolf89MaayanSalton10YotamDrier9

    The Hebrew University-Hadassah COVID-19 diagnosis team

    Show more

    https://doi.org/10.1016/j.cmi.2020.06.009

    Abstract

    Objectives

    Testing for active SARS-CoV-2 infection is a fundamental tool in the public health measures taken to control the COVID-19 pandemic. Due to the overwhelming use of SARS-CoV-2 RT-PCR tests worldwide, availability of test kits has become a major bottleneck, while the need to increase testing throughput only rises. We aim to overcome these challenges by pooling samples together, performing RNA extraction and RT-PCR in pools.

    Methods

    We tested the efficiency and sensitivity of pooling strategies for RNA extraction and RT-PCR detection of SARS-CoV-2. We tested 184 samples both individually and in pools to estimate the effects of pooling. We further implemented Dorfman pooling with a pool size of 8 samples in large-scale clinical tests.

  • 原文来源：https://www.sciencedirect.com/science/article/pii/S1198743X20303499
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