Imaging of gene expression in live cells or tissues has largely relied on light-based strategies detecting fluorescence or luminescence, but poor transmission of light through tissues limits the depth that can be imaged. A new study reports a synthetic biology approach to encode an ultrasound-based gene expression reporter that is applicable to mammalian cells in vitro and in vivo. Credit: Chnit Siri Kan Ti N Cheiynghim/EyeEm/Getty To adopt ultrasound as a robust biomolecular readout, strategie...
Nasal Gene Expression of Angiotensin-Converting Enzyme 2 in Children and Adults
Supinda Bunyavanich, MD, MPH1; Anh Do, PhD2; Alfin Vicencio, MD1
Author Affiliations Article Information
JAMA. Published online May 20, 2020. doi:10.1001/jama.2020.8707
Children account for less than 2% of identified cases of coronavirus disease 2019 (COVID-19).1,2 It is hypothesized that the lower risk among children is due to differential expression of angiotensin-converting enzyme 2 (ACE2),3 the receptor that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) uses for host entry.4 We investigated ACE2 gene expression in the nasal epithelium of children and adults.
We conducted a retrospective examination of nasal epithelium from individuals aged 4 to 60 years encountered within the Mount Sinai Health System, New York, New York, during 2015-2018. Samples were collected from individuals with and without asthma for research on nasal biomarkers of asthma. The study was approved by the Mount Sinai institutional review board. Written informed consent was obtained from participants (or their parents for minors). Nasal epithelium was collected using a cytology brush that was immediately placed in RNA stabilization fluid and stored at −80 °C. RNA was isolated within 6 months. RNA samples were checked for quality and sequenced as a single batch in 2018. Sequence data processing included sequence alignment and normalization of gene expression counts across genes and samples.
Inhibition of Influenza A virus propagation by benzoselenoxanthenes stabilizing TMPRSS2 Gene G-quadruplex and hence down-regulating TMPRSS2 expression
Li-Wen Shen, Man-Qing Qian, Kai Yu, Suresh Narva, Fei Yu, Yan-Ling Wu & Wen Zhang
Scientific Reports volume 10, Article number: 7635 (2020)
Proteolytic cleavage of influenza A virus (IAV) hemagglutinin by host proteases is crucial for virus infectivity and spread. The transmembrane serine protease TMPRSS2 was previously identified as the essential protease that can cleave hemagglutinin of many subtypes of influenza virus and spike protein of coronavirus. Herein, we found that a guanine rich tract, capable of forming intramolecular G-quadruplex in the presence of potassium ions, in the promoter region of human TMPRSS2 gene was quite important for gene transcriptional activity, hence affecting its function.