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《Science，5月20日，SARS-CoV-2 infection protects against rechallenge in rhesus macaques》

  • 来源专题：COVID-19科研动态监测
  • 编译者： xuwenwhlib
  • 发布时间：2020-05-21

  • SARS-CoV-2 infection protects against rechallenge in rhesus macaques

    View ORCID ProfileAbishek Chandrashekar1,*, View ORCID ProfileJinyan Liu1,*, View ORCID ProfileAmanda J. Martinot1,2,*, View ORCID ProfileKatherine McMahan1,*, View ORCID ProfileNoe B. Mercado1,*, View ORCID ProfileLauren Peter1,*, View ORCID ProfileLisa H. Tostanoski1,*, View ORCID ProfileJingyou Yu1,*, View ORCID ProfileZoltan Maliga3, Michael Nekorchuk4, View ORCID ProfileKathleen Busman-Sahay4, View ORCID ProfileMargaret Terry4, View ORCID ProfileLinda M. Wrijil2, View ORCID ProfileSarah Ducat2, David R. Martinez5, View ORCID ProfileCaroline Atyeo3,6, View ORCID ProfileStephanie Fischinger6, View ORCID ProfileJohn S. Burke6, View ORCID ProfileMatthew D. Slein6, View ORCID ProfileLaurent Pessaint7, View ORCID ProfileAlex Van Ry7, View ORCID ProfileJack Greenhouse7, View ORCID ProfileTammy Taylor7, View ORCID ProfileKelvin Blade7, Anthony Cook7, View ORCID ProfileBrad Finneyfrock7, Renita Brown7, Elyse Teow7, Jason Velasco7, View ORCID ProfileRoland Zahn8, View ORCID ProfileFrank Wegmann8, View ORCID ProfilePeter Abbink1, View ORCID ProfileEsther A. Bondzie1, View ORCID ProfileGabriel Dagotto1,3, View ORCID ProfileMakda S. Gebre1,3, Xuan He1, View ORCID ProfileCatherine Jacob-Dolan1,3, View ORCID ProfileNicole Kordana1, Zhenfeng Li1, View ORCID ProfileMichelle A. Lifton1, View ORCID ProfileShant H. Mahrokhian1, View ORCID ProfileLori F. Maxfield1, View ORCID ProfileRamya Nityanandam1, View ORCID ProfileJoseph P. Nkolola1, View ORCID ProfileAaron G. Schmidt6,9, View ORCID ProfileAndrew D. Miller10, View ORCID ProfileRalph S. Baric5, View ORCID ProfileGalit Alter6,9, View ORCID ProfilePeter K. Sorger3, View ORCID ProfileJacob D. Estes4, View ORCID ProfileHanne Andersen7, View ORCID ProfileMark G. Lewis7, View ORCID ProfileDan H. Barouch1,6,9,†

    See all authors and affiliations

    Science 20 May 2020:

    eabc4776

    DOI: 10.1126/science.abc4776

    Abstract

    An understanding of protective immunity to SARS-CoV-2 is critical for vaccine and public health strategies aimed at ending the global COVID-19 pandemic. A key unanswered question is whether infection with SARS-CoV-2 results in protective immunity against re-exposure. We developed a rhesus macaque model of SARS-CoV-2 infection and observed that macaques had high viral loads in the upper and lower respiratory tract, humoral and cellular immune responses, and pathologic evidence of viral pneumonia. Following initial viral clearance, animals were rechallenged with SARS-CoV-2 and showed 5 log10 reductions in median viral loads in bronchoalveolar lavage and nasal mucosa compared with primary infection. Anamnestic immune responses following rechallenge suggested that protection was mediated by immunologic control. These data show that SARS-CoV-2 infection induced protective immunity against re-exposure in nonhuman primates.

  • 原文来源：https://science.sciencemag.org/content/early/2020/05/19/science.abc4776
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    • 来源专题：COVID-19科研动态监测
    • 编译者：zhangmin
    • 发布时间：2020-07-07
    • Primary exposure to SARS-CoV-2 protects against reinfection in rhesus macaques Wei Deng1,*, Linlin Bao1,*, Jiangning Liu1,*, Chong Xiao1,*, Jiayi Liu2,*, Jing Xue1,*, Qi Lv1,*, Feifei Qi1 See all authors and affiliations Science  02 Jul 2020: eabc5343 DOI: 10.1126/science.abc5343 Abstract Coronavirus disease 2019 (COVID-19), which is caused by infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become a global pandemic. It currently remains unclear whether convalescing patients have a risk of reinfection. We generated a rhesus macaque model of SARS-CoV-2 infection that was characterized by interstitial pneumonia and systemic viral dissemination mainly in the respiratory and gastrointestinal tracts. Rhesus macaques reinfected with the identical SARS-CoV-2 strain during the early recovery phase of the initial SARS-CoV-2 infection did not show detectable viral dissemination, clinical manifestations of viral disease, or histopathological changes. Comparing the humoral and cellular immunity between primary infection and rechallenge revealed notably enhanced neutralizing antibody and immune responses. Our results suggest that primary SARS-CoV-2 exposure protects against subsequent reinfection in rhesus macaques.
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  • 《Science，5月20日，DNA vaccine protection against SARS-CoV-2 in rhesus macaques》
    • 来源专题：COVID-19科研动态监测
    • 编译者：xuwenwhlib
    • 发布时间：2020-05-21
    • DNA vaccine protection against SARS-CoV-2 in rhesus macaques View ORCID ProfileJingyou Yu1,*, View ORCID ProfileLisa H. Tostanoski1,*, View ORCID ProfileLauren Peter1,*, View ORCID ProfileNoe B. Mercado1,*, View ORCID ProfileKatherine McMahan1,*, View ORCID ProfileShant H. Mahrokhian1,*, View ORCID ProfileJoseph P. Nkolola1,*, View ORCID ProfileJinyan Liu1,*, Zhenfeng Li1,*, View ORCID ProfileAbishek Chandrashekar1,*, David R. Martinez2, View ORCID ProfileCarolin Loos3, View ORCID ProfileCaroline Atyeo3, View ORCID ProfileStephanie Fischinger3, View ORCID ProfileJohn S. Burke3, View ORCID ProfileMatthew D. Slein3, Yuezhou Chen4, View ORCID ProfileAdam Zuiani4, View ORCID ProfileFelipe J. N. Lelis4, Meghan Travers4, View ORCID ProfileShaghayegh Habibi4, View ORCID ProfileLaurent Pessaint5, View ORCID ProfileAlex Van Ry5, View ORCID ProfileKelvin Blade5, Renita Brown5, Anthony Cook5, View ORCID ProfileBrad Finneyfrock5, View ORCID ProfileAlan Dodson5, Elyse Teow5, Jason Velasco5, View ORCID ProfileRoland Zahn6, View ORCID ProfileFrank Wegmann6, View ORCID ProfileEsther A. Bondzie1, View ORCID ProfileGabriel Dagotto1, View ORCID ProfileMakda S. Gebre1, Xuan He1, View ORCID ProfileCatherine Jacob-Dolan1, Marinela Kirilova1, View ORCID ProfileNicole Kordana1, Zijin Lin1, View ORCID ProfileLori F. Maxfield1, Felix Nampanya1, View ORCID ProfileRamya Nityanandam1, View ORCID ProfileJohn D. Ventura1, View ORCID ProfileHuahua Wan1, View ORCID ProfileYongfei Cai7, View ORCID ProfileBing Chen7,8, View ORCID ProfileAaron G. Schmidt3,8, View ORCID ProfileDuane R. Wesemann4,8, View ORCID ProfileRalph S. Baric2, View ORCID ProfileGalit Alter3,8, View ORCID ProfileHanne Andersen5, View ORCID ProfileMark G. Lewis5, View ORCID ProfileDan H. Barouch1,3,8,† See all authors and affiliations Science 20 May 2020: eabc6284 DOI: 10.1126/science.abc6284 Abstract The global COVID-19 pandemic caused by the SARS-CoV-2 virus has made the development of a vaccine a top biomedical priority. In this study, we developed a series of DNA vaccine candidates expressing different forms of the SARS-CoV-2 Spike (S) protein and evaluated them in 35 rhesus macaques. Vaccinated animals developed humoral and cellular immune responses, including neutralizing antibody titers comparable to those found in convalescent humans and macaques infected with SARS-CoV-2. Following vaccination, all animals were challenged with SARS-CoV-2, and the vaccine encoding the full-length S protein resulted in >3.1 and >3.7 log10 reductions in median viral loads in bronchoalveolar lavage and nasal mucosa, respectively, as compared with sham controls. Vaccine-elicited neutralizing antibody titers correlated with protective efficacy, suggesting an immune correlate of protection. These data demonstrate vaccine protection against SARS-CoV-2 in nonhuman primates.
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