《Nanotechnology in tissue engineering: expanding possibilities with nanoparticles》

  • 来源专题:现代化工
  • 编译者: 武春亮
  • 发布时间:2024-07-01




















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    ACCEPTED MANUSCRIPT




    Nanotechnology in tissue engineering: expanding possibilities with nanoparticles


    Sohrab Sardari1, Ali Hheidari2, Maryam Ghodousi3, Amid Rahi4 and Esmail Pishbin5




    Accepted Manuscript online 28 June 2024
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    DOI 10.1088/1361-6528/ad5cfb

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    Author e-mailsamidrahi@gmail.com
    Author affiliations1
    Iran University of Science and Technology, School of Mechanical Engineering, Tehran, 13114-16846, Iran (the Islamic Republic of)
    2 Department of Mechanical Engineering, Islamic Azad University Science and Research Branch, Department of Mechanical Engineering, Tehran, 1477893855, Iran (the Islamic Republic of)
    3 Department of Mechanical Engineering, The Pennsylvania State University, Department of Mechanical Engineering, University Park, 16802-1503, UNITED STATES
    4 Pathology and Stem Cell Research Center, Kerman University of Medical Sciences, Pathology and Stem Cell Research Center, Kerman, 7616914115, Iran (the Islamic Republic of)
    5 Department of Electrical Engineering and Information Technology, Iranian Research Organization for Science and Technology, Department of Electrical Engineering and Information Technology, Tehran, 87565424, Iran (the Islamic Republic of)

    ORCID iDsAmid Rahi https://orcid.org/0000-0002-9190-5977Esmail Pishbin https://orcid.org/0000-0003-1335-5970


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    Received 29 October 2023
    Revised 4 June 2024
    Accepted 28 June 2024
    Accepted Manuscript online 28 June 2024





















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    10.1088/1361-6528/ad5cfb

    Abstract



    Tissue engineering is a multidisciplinary field that merges engineering, material science, and medical biology in order to develop biological alternatives for repairing, replacing, maintaining, or boosting the functionality of tissues and organs. The ultimate goal of tissue engineering is to create biological alternatives for repairing, replacing, maintaining, or enhancing the functionality of tissues and organs. However, the current landscape of tissue engineering techniques presents several challenges, including a lack of suitable biomaterials, inadequate cell proliferation, limited methodologies for replicating desired physiological structures, and the unstable and insufficient production of growth factors, which are essential for facilitating cell communication and the appropriate cellular responses. Despite these challenges, there has been significant progress made in tissue engineering techniques in recent years. Nanoparticles hold a major role within the realm of nanotechnology due to their unique qualities that change with size. These particles, which provide potential solutions to the issues that are met in tissue engineering, have helped propel nanotechnology to its current state of prominence. Despite substantial breakthroughs in the utilization of nanoparticles over the past two decades, the full range of their potential in addressing the difficulties within tissue engineering remains largely untapped. This is due to the fact that these advancements have occurred in relatively isolated pockets. In the realm of tissue engineering, the purpose of this research is to conduct an in-depth investigation of the several ways in which various types of nanoparticles might be put to use. In addition to this, it sheds light on the challenges that need to be conquered in order to unlock the maximum potential of nanotechnology in this area.




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  • 原文来源:https://iopscience.iop.org/article/10.1088/1361-6528/ad5cfb/meta
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  • 《Production of Cu/Zn Nanoparticles by Pulsed Laser Ablation in Liquids and Sintered Cu/Zn Alloy》

    • 来源专题:现代化工
    • 编译者:武春亮
    • 发布时间:2024-07-25
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Furthermore, it was clarified by SEM EDS that copper and zinc atoms in the nanoalloy were maintained at a ratio of 3:1 in sintered alloy, and that the atoms were spatially uniformly distributed over a wide range in sintered metal. Access through your institution Add to Cart You might also be interested in these eBooks View Preview Info: Periodical: Journal of Nano Research (Volume 83) Pages: 91-108 DOI: https://doi.org/10.4028/p-Bo8Als Citation: Cite this paper Online since: July 2024 Authors: Taku Saiki, Mitsuru Inada Keywords: Brass Powder, Cu/Zn Alloy, High-Entropy Alloys (HEA), Laser Ablation in Liquids, Metal nanoparticles Export: RIS, BibTeX Price: Permissions: Request Permissions Share: - Corresponding Author References [1] Haruyuki Inui, High-Entropy Alloy, Uchidaroukakuho, Tokyo, 2020. Google Scholar [2] S.-H. Joo, J. W. Bae, W.-Y. Park, Y. Shimada, T. Wada, H. S. Kim, A. Takeuchi, T. J. Konno, H. Kato, I. V. 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  • 《Nanoparticles in Autophagy Modulation》

    • 来源专题:现代化工
    • 编译者:武春亮
    • 发布时间:2024-06-19
    • LC3 transfection was conducted using GFP–LC3 Expression Vector (CELL BIOLABS, San Diego, CA, USA, CBA-401). Briefly, experiments were performed after growing at 80–90% cell confluence. Cells were detached by Trypsin-EDTA (0.25%) (GibcoTM, 25200072) and centrifuged at 1200 rpm for 3 min. The supernatant was discarded and resuspended in new media. The cells were collected (0.5 × 10 6 cells) and centrifuged (300 g for 5 min). The supernatant was discarded and washed with DPBS (WELGENE, Busan, Republic of Korea, LB 001-02). After centrifuge, cells were resuspended with DPBS. Then, 1 μg GFP-LC3 DNA was added to the cells. Transfection was performed with the NeonTM Transfection System, which is an electroporation system (InvitrogenTM, Carlsbad, CA, USA). After transfection, the cells were seeded to a 6-well plate without antibiotic. Selection cells were proceeded with GeneticinTM Selective Antibiotic (G418 Sulfate; GibcoTM, 10131035). G418 was treated at 800 μg/mL for 3 days and changed to a decreased concentration (200 μg/mL).