登录
 机构网站
  1. 首页
  2. 情报产品
  3. 快讯详情

《IJID，6月12日，Multiple assays in a real-time RT-PCR SARS-CoV-2 panel can mitigate the risk of loss of sensitivity by new genomic variants during the COVID-19 outbreak》

  • 来源专题：COVID-19科研动态监测
  • 编译者： zhangmin
  • 发布时间：2020-06-14

  • Multiple assays in a real-time RT-PCR SARS-CoV-2 panel can mitigate the risk of loss of sensitivity by new genomic variants during the COVID-19 outbreak
    Author links open overlay panelAuthors: LuisPenarrubiaaMariaRuizaRobertoPorcoaSonia N.RaobMartíJuanola-FalgaronaaDavideManisserocMartaLópez-FontanalsaJosepParejaa
    Show more
    https://doi.org/10.1016/j.ijid.2020.06.027

    Abstract
    Objectives
    In this study, five SARS-CoV-2 PCR assay panels were evaluated against the accumulated genetic variability of the virus to assess the effect on sensitivity of the individual assays.

    Design or methods
    As of week 21, 2020, the complete set of available SARS-CoV-2 genomes from GISAID and GenBank databases were used in this study. SARS-CoV-2 primer sequences from publicly available panels (WHO, CDC, NMDC, and HKU) and QIAstat-Dx were included in the alignment, and accumulated genetic variability affecting any oligonucleotide annealing was annotated.

  • 原文来源：https://www.sciencedirect.com/science/article/pii/S120197122030463X
38浏览量
原文链接
相关报告

  • 《6月12日_RT-PCR多次检测SARS-CoV-2可减少由基因组变异引起的检测敏感性丢失》
    • 来源专题：COVID-19科研动态监测
    • 编译者：xuwenwhlib
    • 发布时间：2020-06-14
    • 信息名称：RT-PCR多次检测SARS-CoV-2可减少由基因组变异引起的检测敏感性丢失 1.时间：2020年6月12日 2.机构或团队：西班牙STAT-Dx公司、美国QIAGEN公司 3.事件概要： 西班牙STAT-Dx公司等机构在International Journal of Infectious Diseases发表论文“Multiple assays in a real-time RT-PCR SARS-CoV-2 panel can mitigate the risk of loss of sensitivity by new genomic variants during the COVID-19 outbreak”。 文章针对病毒累积的遗传变异对5个SARS-CoV-2 PCR检测面板进行了评估，以评估对单个检测的敏感性。在2020年第21周，文章使用了来自GISAID和GenBank数据库的完整的SARS-CoV-2基因组，使用了来自公开的平台（WHO、CDC、NMDC和HKU）和QIAstat-Dx的SARS-CoV-2引物序列进行比对，注释了影响寡核苷酸退火的累积遗传变异。分析结果显示，共有11,627个基因组（34.38%）包含单个突变，影响PCR试验的退火，8,773（25.94%）基因组的变异被认为是高风险，而另外2,854（8.43%）基因组表现为低频率的单突变，预计不会对敏感性产生影响。在QIAstat-Dx面板中，99.11%的基因组匹配了100%的寡核苷酸覆盖率，并且在体外测试了关键变异，证实敏感性没有损失。 4.附件： 原文链接：https://www.sciencedirect.com/science/article/pii/S120197122030463X
    33浏览量
    原文链接

  • 《Science，6月8日，Genomic surveillance reveals multiple introductions of SARS-CoV-2 into Northern California》
    • 来源专题：COVID-19科研动态监测
    • 编译者：xuwenwhlib
    • 发布时间：2020-06-09
    • REPORT Genomic surveillance reveals multiple introductions of SARS-CoV-2 into Northern California Eva W. Cheung, MD1; Philip Zachariah, MD, MS1; Mark Gorelik, MD Science 08 Jun 2020: eabb9263 DOI: 10.1126/science.abb9263 Abstract The COVID-19 pandemic caused by the novel coronavirus SARS-CoV-2 has spread globally, with >52,000 cases in California as of May 4, 2020. Here we investigate the genomic epidemiology of SARS-CoV-2 in Northern California from late January to mid-March 2020, using samples from 36 patients spanning 9 counties and the Grand Princess cruise ship. Phylogenetic analyses revealed the cryptic introduction of at least 7 different SARS-CoV-2 lineages into California, including epidemic WA1 strains associated with Washington State, with lack of a predominant lineage and limited transmission between communities. Lineages associated with outbreak clusters in 2 counties were defined by a single base substitution in the viral genome. These findings support contact tracing, social distancing, and travel restrictions to contain SARS-CoV-2 spread in California and other states.
    57浏览量
    原文链接