登录
 机构网站
  1. 首页
  2. 情报产品
  3. 快讯详情

《MedRxiv，3月9日，Sensitive one-step isothermal detection of pathogen-derived RNAs》

  • 来源专题：COVID-19科研动态监测
  • 编译者： zhangmin
  • 发布时间：2020-03-10

  • Sensitive one-step isothermal detection of pathogen-derived RNAs

    Chang Ha Woo, Sungho Jang, Giyoung Shin, Gyoo Yeol Jung, Jeong Wook Lee

    doi: https://doi.org/10.1101/2020.03.05.20031971

    Abstract

    The recent outbreaks of Ebola, Zika, MERS, and SARS-CoV-2 (2019-nCoV) require fast, simple, and sensitive onsite nucleic acid diagnostics that can be developed rapidly to prevent the spread of diseases. We have developed a SENsitive Splint-based one-step isothermal RNA detection (SENSR) method for rapid and straightforward onsite detection of pathogen RNAs with high sensitivity and specificity. SENSR consists of two simple enzymatic reactions: a ligation reaction by SplintR ligase and subsequent transcription by T7 RNA polymerase. The resulting transcript forms an RNA aptamer that induces fluorescence. Here, we demonstrate that SENSR is an effective and highly sensitive method for the detection of the current epidemic pathogen, severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2).

    *注，本文为预印本论文手稿，是未经同行评审的初步报告，其观点仅供科研同行交流，并不是结论性内容，请使用者谨慎使用.

  • 原文来源：https://www.medrxiv.org/content/10.1101/2020.03.05.20031971v1
140浏览量
原文链接
相关报告

  • 《3月9日_病原体RNA的高灵敏度一步式等温检测》
    • 来源专题：COVID-19科研动态监测
    • 编译者：zhangmin
    • 发布时间：2020-03-10
    • 1.时间：2020年3月9日 2.机构或团队：浦项科技大学 3.事件概要： 浦项科技大学的科研人员在medRxiv预印版平台发表论文“Sensitive one-step isothermal detection of pathogen-derived RNAs”，介绍了病原体RNA的高灵敏度一步式等温检测方法。 文章指出，埃博拉、寨卡病毒、MERS和SARS-CoV-2疫情（2019-nCoV）要求快速、简单和灵敏的现场核酸诊断，以便快速检测来预防疾病传播。科研人员开发了基于敏感夹板的一步式等温RNA检测（SENsitive Splint-based one-step isothermal RNA detection ，SENSR）方法，可快速、直接地现场检测具有高灵敏度和特异性的病原体RNA。SENSR由两个简单的酶促反应组成：通过SplintR连接酶进行的连接反应和随后通过T7 RNA聚合酶进行的转录，所得的转录物形成诱导荧光的RNA适体。文章中，科研人员证明了SENSR是检测当前流行病原体SARS-CoV-2的有效且高度灵敏的方法。另外还显示该平台可以扩展到检测其他五种病原体。总体而言，SENSR是一种分子诊断方法，可以快速开发应用于需要高灵敏度、特异性和检测时间短的场合。 4.附件： 原文链接： https://www.medrxiv.org/content/10.1101/2020.03.05.20031971v1.full.pdf
    70浏览量
    原文链接

  • 《MedRxiv，3月20日，Reverse transcription loop-mediated isothermal amplification combined with nanoparticles-based biosensor for diagnosis of COVID-19》
    • 来源专题：COVID-19科研动态监测
    • 编译者：zhangmin
    • 发布时间：2020-03-22
    • Reverse transcription loop-mediated isothermal amplification combined with nanoparticles-based biosensor for diagnosis of COVID-19 Xiong Zhu, Xiaoxia Wang, Limei Han, Ting Chen, Licheng Wang, Huan Li, Sha Li, Lvfen He, Xiaoying Fu, Shaojin Chen, Xing Mei, Hai Chen, Yi Wang doi: https://doi.org/10.1101/2020.03.17.20037796 Abstract Given the scale and rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, known as 2019-nCov) infection (COVID-19), the ongoing global SARS-CoV-2 outbreak has become a huge public health issue. Rapid and precise diagnostic methods are thus immediately needed for diagnosing COVID-19, providing timely treatment and facilitating infection control. A one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) coupled with nanoparticles-based biosensor (NBS) assay (RT-LAMP-NBS) was successfully established for rapidly and accurately diagnosing COVID-19. *注，本文为预印本论文手稿，是未经同行评审的初步报告，其观点仅供科研同行交流，并不是结论性内容，请使用者谨慎使用.
    132浏览量
    原文链接