Reverse transcription loop-mediated isothermal amplification combined with nanoparticles-based biosensor for diagnosis of COVID-19
Xiong Zhu, Xiaoxia Wang, Limei Han, Ting Chen, Licheng Wang, Huan Li, Sha Li, Lvfen He, Xiaoying Fu, Shaojin Chen, Xing Mei, Hai Chen, Yi Wang
doi: https://doi.org/10.1101/2020.03.17.20037796
Abstract
Given the scale and rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, known as 2019-nCov) infection (COVID-19), the ongoing global SARS-CoV-2 outbreak has become a huge public health issue. Rapid and precise diagnostic methods are thus immediately needed for diagnosing COVID-19, providing timely treatment and facilitating infection control. A one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) coupled with nanoparticles-based biosensor (NBS) assay (RT-LAMP-NBS) was successfully established for rapidly and accurately diagnosing COVID-19.
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