Design of multi epitope-based peptide vaccine against E protein of human 2019-nCoV: An immunoinformatics approach
Miysaa I. Abdelmageed, Abdelrahman Hamza Abdelmoneim Sr., Mujahed I. Mustafa Sr., Nafisa M. Elfadol, Naseem S. Murshed, Shaza W. Shantier, Abdelrafie M. Makhawi
doi: https://doi.org/10.1101/2020.02.04.934232
Abstract
Background: on the late December 2019, a new endemic has been spread across Wuhan City, China; within a few weeks, a novel coronavirus designated as 2019 novel coronavirus (2019-nCoV) was announced by the World Health Organization (WHO). In late January 2020, WHO declared the outbreak a public-health emergency of international concern due to the rapid spreading and increasing worldwide. There is no vaccine or approved treatment for this emerging infection; therefore, the objective of this paper is to design a multi epitope peptide vaccine against 2019-nCoV using immunoinformatics approach. Method: We will highlight a technique facilitating the combination of immunoinformatics approach with comparative genomic approach to determine our potential target for designing the T cell epitopes-based peptide vaccine using the envelope protein of 2019-nCoV as a target. Results: Extensive mutations, insertion and deletion were discovered with comparative sequencing in 2019-nCoV strain; in addition, 10 MHC1 and MHC2 related peptides were promising candidates for vaccine design with adequate world population coverage of 88.5% and 99.99% respectively. Conclusion: T cell epitopes-based peptide vaccine was designed for 2019-nCoV using envelope protein as an immunogenic target; nevertheless, the proposed T cell epitopes-based peptide vaccine rapidly needs to validates clinically to ensure its safety and immunogenic profile to assist on stopping this epidemic before it leads to devastating global outbreaks.
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