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《BioRxiv，3月30日，Orthogonal genome-wide screenings in bat cells identify MTHFD1 as a target of broad antiviral therapy》

  • 来源专题：COVID-19科研动态监测
  • 编译者： zhangmin
  • 发布时间：2020-03-31

  • Orthogonal genome-wide screenings in bat cells identify MTHFD1 as a target of broad antiviral therapy

    Danielle E Anderson, Jin Cui, Qian Ye, Baoying Huang, Wenhong Zu, Jing Gong, Weiqiang Liu, So Young Kim, Biao Guo Yan, Kristmundur Sigmundsson, Xiao Fang Lim, Fei Ye, Peihua Niu, Xuming Zhou, Wenjie Tan, Lin-Fa Wang, Xu Tan

    doi: https://doi.org/10.1101/2020.03.29.014209

    Abstract

    Bats are responsible for the zoonotic transmission of several major viral diseases including the 2003 SARS outbreak and the ongoing COVID-19 pandemic. While bat genomic sequencing studies have revealed characteristic adaptations of the innate immune system, functional genomic studies are urgently needed to provide a foundation for the molecular dissection of the tolerance of viral infections in bats. Here we report the establishment and screening of genome-wide RNAi library and CRISPR library for the model megabat, Pteropus Alecto. We used the complementary RNAi and CRISPR libraries to interrogate Pteropus Alecto cells for infection with two different viruses, mumps virus and Influenza A virus, respectively. Screening results converged on the endocytosis pathway and the protein secretory pathway as required for both viral infections.

    *注，本文为预印本论文手稿，是未经同行评审的初步报告，其观点仅供科研同行交流，并不是结论性内容，请使用者谨慎使用.

  • 原文来源：https://www.biorxiv.org/content/10.1101/2020.03.29.014209v1
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