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《Cell Research，7月1日，Mining of epitopes on spike protein of SARS-CoV-2 from COVID-19 patients》

  • 来源专题：COVID-19科研动态监测
  • 编译者： zhangmin
  • 发布时间：2020-07-07

  • Mining of epitopes on spike protein of SARS-CoV-2 from COVID-19 patients
    Bao-zhong Zhang, Ye-fan Hu, Lin-lei Chen, Thomas Yau, Yi-gang Tong, Jing-chu Hu, Jian-piao Cai, Kwok-Hung Chan, Ying Dou, Jian Deng, Xiao-lei Wang, Ivan Fan-Ngai Hung, Kelvin Kai-Wang To, Kwok Yung Yuen & Jian-Dong Huang
    Cell Research (2020)

    The ongoing coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is a serious threat to global public health, and is imposing severe burdens on human society. Several candidate vaccines against SARS-CoV-2 are now undergoing clinical trials. The Spike (S) protein of SARS-CoV-2 is widely considered as a promising antigen. However, limited information about the protective immune response against SARS-CoV-2 has been reported.1 In vivo or in natura data of the immune response in patients, including major immune responses to S protein, are currently lacking. The development of effective and safe vaccines against SARS-CoV-2 is urgently needed because of some potential adverse events including antibody-dependent enhancement (ADE),2 which might be difficult to avoid in current vaccine designs. Therefore, it is important to mine serological information from COVID-19 patients. In this study, we analysed the correlation between S- or Nucleocapsid (N) protein-specific antibody levels and neutralizing antibody tires. Furthermore, we aimed to identify linear B cell linear immunodominant (ID) sites on the S protein by Pepscan analysis with a series of overlapped peptides against the sera from COVID-19 patients.

  • 原文来源：https://www.nature.com/articles/s41422-020-0366-x
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  • 《Nature，6月1日，Two linear epitopes on the SARS-CoV-2 spike protein that elicit neutralising antibodies in COVID-19 patients》
    • 来源专题：COVID-19科研动态监测
    • 编译者：zhangmin
    • 发布时间：2020-06-02
    • Two linear epitopes on the SARS-CoV-2 spike protein that elicit neutralising antibodies in COVID-19 patients Chek Meng Poh, Guillaume Carissimo, Bei Wang, Siti Naqiah Amrun, Cheryl Yi-Pin Lee, Rhonda Sin-Ling Chee, Siew-Wai Fong, Nicholas Kim-Wah Yeo, Wen-Hsin Lee, Anthony Torres-Ruesta, Yee-Sin Leo, Mark I-Cheng Chen, Seow-Yen Tan, Louis Yi Ann Chai, Shirin Kalimuddin, Shirley Seah Gek Kheng, Siew-Yee Thien, Barnaby Edward Young, David C. Lye, Brendon John Hanson, Cheng-I Wang, Laurent Renia & Lisa F. P. Ng Nature Communications volume 11, Article number: 2806 (2020) Abstract Given the ongoing SARS-CoV-2 pandemic, identification of immunogenic targets against the coronavirus spike glycoprotein will provide crucial advances towards the development of sensitive diagnostic tools and potential vaccine candidate targets. In this study, using pools of overlapping linear B-cell peptides, we report two IgG immunodominant regions on SARS-CoV-2 spike glycoprotein that are recognised by sera from COVID-19 convalescent patients. Notably, one is specific to SARS-CoV-2, which is located in close proximity to the receptor binding domain. The other region, which is localised at the fusion peptide, could potentially function as a pan-SARS target. Functionally, antibody depletion assays demonstrate that antibodies targeting these immunodominant regions significantly alter virus neutralisation capacities. Taken together, identification and validation of these neutralising B-cell epitopes will provide insights towards the design of diagnostics and vaccine candidates against this high priority coronavirus.
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  • 《BioRxiv，3月31日，Potent neutralizing antibodies in the sera of convalescent COVID-19 patients are directed against conserved linear epitopes on the SARS-CoV-2 spike protein》
    • 来源专题：COVID-19科研动态监测
    • 编译者：zhangmin
    • 发布时间：2020-04-01
    • Potent neutralizing antibodies in the sera of convalescent COVID-19 patients are directed against conserved linear epitopes on the SARS-CoV-2 spike protein Chek Meng Poh, Guillaume Carissimo, Wang Bei, Siti Naqiah Amrun, Cheryl Yi-Pin Lee, Rhonda Sin-Ling Chee, Nicholas Kim-Wah Yeo, Wen-Hsin Lee, Yee-Sin Leo, Mark I-Cheng Chen, Seow-Yen Tan, Louis Yi Ann Chai, Shirin Kalimuddin, Siew-Yee Thien, Barnaby Edward Young, David C. Lye, Cheng-I Wang, Laurent Renia, Lisa FP Ng doi: https://doi.org/10.1101/2020.03.30.015461 Abstract The ongoing SARS-CoV-2 pandemic demands rapid identification of immunogenic targets for the design of efficient vaccines and serological detection tools. In this report, using pools of overlapping linear peptides and functional assays, we present two immunodominant regions on the spike glycoprotein that were highly recognized by neutralizing antibodies in the sera of COVID-19 convalescent patients. One is highly specific to SARS-CoV-2, and the other is a potential pan-coronavirus target. *注，本文为预印本论文手稿，是未经同行评审的初步报告，其观点仅供科研同行交流，并不是结论性内容，请使用者谨慎使用.
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