Rapid Detection of SARS-CoV-2 Using Reverse transcription RT-LAMP method
Weihua Yang, Xiaofei Dang, Qingxi Wang, Mingjie Xu, Qianqian Zhao, Yunying Zhou, Huailong Zhao, Li Wang, Yihui Xu, Jun Wang, Shuyi Han, Min Wang, Fengyan Pei, Yunshan Wang
doi: https://doi.org/10.1101/2020.03.02.20030130
Abstract
Corona Virus Disease 2019 (COVID-19) is a recently emerged life-threatening disease caused by SARS-CoV-2. Real- time fluorescent PCR (RT-PCR) is the clinical standard for SARS-CoV-2 nucleic acid detection. To detect SARS-CoV-2 early and control the disease spreading on time, a faster and more convenient method for SARS-CoV-2 nucleic acid detecting, RT-LAMP method (reverse transcription loop-mediated isothermal amplification) was developed. RNA reverse transcription and nucleic acid amplification were performed in one step at 63 ℃ isothermal conditions, and the results can be obtained within 30 minutes.
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