《MedRixv,2月22日,(第2版更新)Longitudinal characteristics of lymphocyte responses and cytokine profiles in the peripheral blood of SARS-CoV-2 infected patients》

  • 来源专题:COVID-19科研动态监测
  • 编译者: zhangmin
  • 发布时间:2020-02-23
  • Longitudinal characteristics of lymphocyte responses and cytokine profiles in the peripheral blood of SARS-CoV-2 infected patients

    Jing Liu, Sumeng Li, Jia Liu, Boyun Liang, Xiaobei Wang, Hua Wang, Wei Li, Qiaoxia Tong, Jianhua Yi, Lei Zhao, Lijuan Xiong, Chunxia Guo, Jin Tian, Jinzhuo Luo, Jinghong Yao, Ran Pang, Hui Shen, Cheng Peng, Ting Liu, Qian Zhang, Jun Wu, Ling Xu, Sihong Lu, Baoju Wang, Zhihong Weng, Chunrong Han, Huabing Zhu, Ruxia Zhou, Helong Zhou, Xiliu Chen, Pian Ye, Bin Zhu, Shengsong He, Yongwen He, Shenghua Jie, Ping Wei, Jianao Zhang, Yinping Lu, Weixian Wang, Li Zhang, Ling Li, Fengqin Zhou, Jun Wang, Ulf Dittmer, Mengji Lu, Yu Hu, Dongliang Yang, Xin Zheng

    doi: https://doi.org/10.1101/2020.02.16.20023671

    Abstract

    Background: The dynamic changes of lymphocyte subsets and cytokines profiles of patients with novel coronavirus disease (COVID-19) and their correlation with the disease severity remain unclear. Method: Peripheral blood samples were longitudinally collected from 40 confirmed COVID-19 patients and examined for lymphocyte subsets by flow cytometry and cytokine profiles by specific immunoassays. Findings: Of the 40 COVID-19 patients enrolled, 13 severe cases showed significant and sustained decreases in lymphocyte counts but increases in neutrophil counts than 27 mild cases. Further analysis demonstrated significant decreases in the counts of T cells, especially CD8 + T cells, as well as increases in IL-6, IL-10, IL-2 and IFN-γ levels in the peripheral blood in the severe cases compared to those in the mild cases. T cell counts and cytokine levels in severe COVID-19 patients who survived the disease gradually recovered at later time points to levels that were comparable to those of the mild cases. Moreover, the neutrophil-to-CD8+ T cell ratio (N8R) were identified as the most powerful prognostic factor affecting the prognosis for severe COVID-19. Conclusion: The degree of lymphopenia and a proinflammatory cytokine storm is higher in severe COVID-19 patients than in mild cases, and is associated with the disease severity. N8R may serve as a useful prognostic factor for early identification of severe COVID-19 cases.

    *注,本文为预印本论文手稿,是未经同行评审的初步报告,其观点仅供科研同行交流,并不是结论性内容,请使用者谨慎使用.

  • 原文来源:https://www.medrxiv.org/content/10.1101/2020.02.16.20023671v2
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