登录
 机构网站
  1. 首页
  2. 情报产品
  3. 快讯详情

《MedRxiv，5月17日，（第2版更新）Saliva is less sensitive than nasopharyngeal swabs for COVID-19 detection in the community setting》

  • 来源专题：COVID-19科研动态监测
  • 编译者： zhangmin
  • 发布时间：2020-05-18

  • Saliva is less sensitive than nasopharyngeal swabs for COVID-19 detection in the community setting

    David Becker, Efren Sandoval, Aakash Amin, Peter De Hoff, Amberly Diets, Nicole Leonetti, Yan Wei Lim, Christie Elliott, Louise Laurent, View ORCID ProfileJoseph Grzymski, View ORCID ProfileJames Lu

    doi: https://doi.org/10.1101/2020.05.11.20092338

    Abstract

    The use of saliva collection for SARS-CoV-2 diagnostics in the ambulatory setting provides several advantages when compared to nasopharyngeal swabs (NPS), including ease of self-collection and reduced use of personal protective equipment (PPE). In addition saliva collection could be advantageous in advising if a convalescent patient is able to return to work after a period of self-quarantine. We investigated the utility of saliva collection in the community setting at Renown Health in a prospective Diagnostic Cohort of 88 patients and in a Convalescent Cohort of 24 patients. In the Diagnostic Cohort, we find that saliva collection has reduced sensitivity (~30% less) relative than NPS. And in our convalescent cohort of patients greater than 8 days and less than 21 days from first symptom, we find that saliva has ~ 50% sensitivity relative to NPS. Our results suggest that rigorous studies in the intended populations should be performed before large-scale screening using saliva as the test matrix is initiated.

    *注，本文为预印本论文手稿，是未经同行评审的初步报告，其观点仅供科研同行交流，并不是结论性内容，请使用者谨慎使用.

  • 原文来源：https://www.medrxiv.org/content/10.1101/2020.05.11.20092338v2
42浏览量
原文链接
相关报告

  • 《Nature，2月4日，Saliva is more sensitive than nasopharyngeal or nasal swabs for diagnosis of asymptomatic and mild COVID-19 infection》
    • 来源专题：COVID-19科研动态监测
    • 编译者：zhangmin
    • 发布时间：2021-02-25
    • Saliva is more sensitive than nasopharyngeal or nasal swabs for diagnosis of asymptomatic and mild COVID-19 infection Alvin Kuo Jing Teo, Yukti Choudhury, Iain Beehuat Tan, Chae Yin Cher, Shi Hao Chew, Zi Yi Wan, Lionel Tim Ee Cheng, Lynette Lin Ean Oon, Min Han Tan, Kian Sing Chan & Li Yang Hsu Scientific Reports volume 11, Article number: 3134 (2021) Abstract We aimed to test the sensitivity of naso-oropharyngeal saliva and self-administered nasal (SN) swab compared to nasopharyngeal (NP) swab for COVID-19 testing in a large cohort of migrant workers in Singapore. We also tested the utility of next-generation sequencing (NGS) for diagnosis of COVID-19. Saliva, NP and SN swabs were collected from subjects who presented with acute respiratory infection, their asymptomatic roommates, and prior confirmed cases who were undergoing isolation at a community care facility in June 2020. All samples were tested using RT-PCR. SARS-CoV-2 amplicon-based NGS with phylogenetic analysis was done for 30 samples. We recruited 200 subjects, of which 91 and 46 were tested twice and thrice respectively. In total, 62.0%, 44.5%, and 37.7% of saliva, NP and SN samples were positive. Cycle threshold (Ct) values were lower during the earlier period of infection across all sample types. The percentage of test-positive saliva was higher than NP and SN swabs. We found a strong correlation between viral genome coverage by NGS and Ct values for SARS-CoV-2. Phylogenetic analyses revealed Clade O and lineage B.6 known to be circulating in Singapore. We found saliva to be a sensitive and viable sample for COVID-19 diagnosis.
    71浏览量
    原文链接

  • 《MedRxiv，5月10日，（第2版更新）Pre-Existing Traits Associated with Covid-19 Illness Severity》
    • 来源专题：COVID-19科研动态监测
    • 编译者：zhangmin
    • 发布时间：2020-05-11
    • Pre-Existing Traits Associated with Covid-19 Illness Severity Joseph E. Ebinger, Natalie Achamallah, Hongwei Ji, Brian L. Claggett, Nancy Sun, Patrick Botting, Trevor-Trung Nguyen, Eric Luong, Elizabeth H. Kim, Eunice Park, Yunxian Liu, Ryan Rosenberry, Yuri Matusov, Steven Zhao, Isabel Pedraza, Tanzira Zaman, Michael Thompson, Koen Raedschelders, Anders H. Berg, Jonathan D. Grein, Paul W. Noble, Sumeet S. Chugh, C. Noel Bairey Merz, Eduardo Marbán, Jennifer E. Van Eyk, Scott D. Solomon, Christine M. Albert, Peter Chen, Susan Cheng doi: https://doi.org/10.1101/2020.04.29.20084533 Abstract Background. Certain individuals, when infected by SARS-CoV-2, tend to develop the more severe forms of Covid-19 illness for reasons that remain unclear. Methods. We studied N=442 patients who presented with laboratory confirmed Covid-19 illness to our U.S. metropolitan healthcare system. We curated data from the electronic health record, and used multivariable logistic regression to examine the association of pre-existing traits with a Covid-19 illness severity defined by level of required care: need for hospital admission, need for intensive care, and need for intubation. Results. Of all patients studied, 48% required hospitalization, 17% required intensive care, and 12% required intubation. In multivariable-adjusted analyses, patients requiring a higher levels of care were more likely to be older (OR 1.5 per 10 years, P<0.001), male (OR 2.0, P=0.001), African American (OR 2.1, P=0.011), obese (OR 2.0, P=0.021), with diabetes mellitus (OR 1.8, P=0.037), and with a higher comorbidity index (OR 1.8 per SD, P<0.001). Several clinical associations were more pronounced in younger compared to older patients (Pinteraction<0.05). Of all hospitalized patients, males required higher levels of care (OR 2.5, P=0.003) irrespective of age, race, or morbidity profile. *注，本文为预印本论文手稿，是未经同行评审的初步报告，其观点仅供科研同行交流，并不是结论性内容，请使用者谨慎使用.
    147浏览量
    原文链接