CRISPR–Cas12-based detection of SARS-CoV-2
James P. Broughton, Xianding Deng, Guixia Yu, Clare L. Fasching, Venice Servellita, Jasmeet Singh, Xin Miao, Jessica A. Streithorst, Andrea Granados, Alicia Sotomayor-Gonzalez, Kelsey Zorn, Allan Gopez, Elaine Hsu, Wei Gu, Steve Miller, Chao-Yang Pan, Hugo Guevara, Debra A. Wadford, Janice S. Chen & Charles Y. Chiu
Nature Biotechnology (2020)
Abstract
An outbreak of betacoronavirus severe acute respiratory syndrome (SARS)-CoV-2 began in Wuhan, China in December 2019. COVID-19, the disease associated with SARS-CoV-2 infection, rapidly spread to produce a global pandemic. We report development of a rapid (<40 min), easy-to-implement and accurate CRISPR–Cas12-based lateral flow assay for detection of SARS-CoV-2 from respiratory swab RNA extracts. We validated our method using contrived reference samples and clinical samples from patients in the United States, including 36 patients with COVID-19 infection and 42 patients with other viral respiratory infections.
