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《Cell，6月23日，Structures of human antibodies bound to SARS-CoV-2 spike reveal common epitopes and recurrent features of antibodies》

  • 来源专题：COVID-19科研动态监测
  • 编译者： zhangmin
  • 发布时间：2020-06-24

  • Structures of human antibodies bound to SARS-CoV-2 spike reveal common epitopes and recurrent features of antibodies

    Christopher O. Barnes

    Anthony P. West Jr.

    Kathryn E. Huey-Tubman

    Davide F. Robbiani 7

    Michel C. Nussenzweig

    Pamela J. Bjorkman

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    Open AccessPublished:June 23, 2020DOI:https://doi.org/10.1016/j.cell.2020.06.025

    Summary

    Neutralizing antibody responses to coronaviruses mainly target the receptor-binding domain (RBD) of the trimeric spike. Here, we characterized polyclonal IgGs and Fabs from COVID-19 convalescent individuals for recognition of coronavirus spikes. Plasma IgGs differed in their focus on RBD epitopes, recognition of alpha- and beta-coronaviruses, and contributions of avidity to increased binding/neutralization of IgGs over Fabs. Using electron microscopy, we examined specificities of polyclonal plasma Fabs, revealing recognition of both S1A and RBD epitopes on SARS-CoV-2 spike. Moreover, a 3.4Å cryo-EM structure of a neutralizing monoclonal Fab-spike complex revealed an epitope that blocks ACE2 receptor binding. Modeling based on these structures suggested different potentials for inter-spike crosslinking by IgGs on viruses and that characterized IgGs would not be affected by identified SARS-CoV-2 spike mutations. Overall, our studies structurally define a recurrent anti-SARS-CoV-2 antibody class derived from VH3-53/VH3-66 and similarity to a SARS-CoV VH3-30 antibody, providing criteria for evaluating vaccine-elicited antibodies.

  • 原文来源：https://www.cell.com/cell/fulltext/S0092-8674(20)30757-1
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