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《CELL，4月8日，The architecture of SARS-CoV-2 transcriptome》

  • 来源专题：COVID-19科研动态监测
  • 编译者： xuwenwhlib
  • 发布时间：2020-04-09

  • The architecture of SARS-CoV-2 transcriptome

    Authors

    Dongwan Kim1,2, Joo-Yeon Lee3

    , Jeong-Sun Yang3

    , Jun Won Kim3

    , V. Narry Kim1,2,4,*, and

    Hyeshik Chang1,2,*

    DOI: 10.1016/j.cell.2020.04.011

    SARS-CoV-2 is a betacoronavirus responsible for the COVID-19 pandemic. Although the

    SARS-CoV-2 genome was reported recently, its transcriptomic architecture is unknown.

    Utilizing two complementary sequencing techniques, we here present a high-resolution map

    of the SARS-CoV-2 transcriptome and epitranscriptome. DNA nanoball sequencing shows

    that the transcriptome is highly complex owing to numerous discontinuous transcription

    events. In addition to the canonical genomic and 9 subgenomic RNAs, SARS-CoV-2

    produces transcripts encoding unknown ORFs with fusion, deletion, and/or frameshift. Using

    nanopore direct RNA sequencing, we further find at least 41 RNA modification sites on viral

    transcripts, with the most frequent motif, AAGAA. Modified RNAs have shorter poly(A) tails

    than unmodified RNAs, suggesting a link between the modification and the 3′ tail. Functional

    investigation of the unknown transcripts and RNA modifications discovered in this study will

    open new directions to our understanding of the life cycle and pathogenicity of SARS-CoV-2.

  • 原文来源：https://www.cell.com/pb-assets/products/coronavirus/CELL_CELL-D-20-00765.pdf
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