登录
 机构网站
  1. 首页
  2. 情报产品
  3. 快讯详情

《BioRxiv，3月14日，SARS-CoV-2 receptor ACE2 and TMPRSS2 are predominantly expressed in a transient secretory cell type in subsegmental bronchial branches》

  • 来源专题：COVID-19科研动态监测
  • 编译者： zhangmin
  • 发布时间：2020-03-15

  • SARS-CoV-2 receptor ACE2 and TMPRSS2 are predominantly expressed in a transient secretory cell type in subsegmental bronchial branches

    Soeren Lukassen, Robert Lorenz Chua, Timo Trefzer, Nicolas C Kahn, Marc A Schneider, Thomas Muley, Hauke Winter, Michael Meister, Carmen Veith, Agnes W Boots, Bianca P Hennig, Michael Kreuter, Christian Conrad, Roland Eils

    doi: https://doi.org/10.1101/2020.03.13.991455

    Abstract

    The SARS-CoV-2 pandemic affecting the human respiratory system severely challenges public health and urgently demands for increasing our understanding of COVID-19 pathogenesis, especially host factors facilitating virus infection and replication. SARS-CoV-2 was reported to enter cells via binding to ACE2, followed by its priming by TMPRSS2. Here, we investigate ACE2 and TMPRSS2 expression levels and their distribution across cell types in lung tissue (twelve donors, 39,778 cells) and in cells derived from subsegmental bronchial branches (four donors, 17,521 cells) by single nuclei and single cell RNA sequencing, respectively.

    *注，本文为预印本论文手稿，是未经同行评审的初步报告，其观点仅供科研同行交流，并不是结论性内容，请使用者谨慎使用.

  • 原文来源：https://www.biorxiv.org/content/10.1101/2020.03.13.991455v1
101浏览量
原文链接
相关报告

  • 《3月14日_SARS-CoV-2受体ACE2和TMPRSS2主要在节段性支气管分支中的瞬时分泌细胞中表达》
    • 来源专题：COVID-19科研动态监测
    • 编译者：xuwenwhlib
    • 发布时间：2020-03-15
    • 信息名称：SARS-CoV-2受体ACE2和TMPRSS2主要在节段性支气管分支中的瞬时分泌细胞中表达 1.时间：2020年3月14日 2.机构或团队：德国柏林大学医学院 3.事件概要： 德国柏林大学医学院在bioRxiv预印本平台发表论文“SARS-CoV-2 receptor ACE2 and TMPRSS2 are predominantly expressed in a transient secretory cell type in subsegmental bronchial branches”。 文章指出，SARSCoV-2通过与ACE2结合进入细胞，然后由TMPRSS2引发。研究人员通过单核和单细胞RNA测序分别研究了肺组织（十二个样本，39,778个细胞）和细分支气管分支细胞（四个样本，17,521个细胞）中ACE2和TMPRSS2的表达水平及其在细胞类型中的分布。结果显示，尽管TMPRSS2在两种组织中均表达，但在节段性支气管分支中，ACE2主要以瞬时分泌细胞类型表达。有趣的是，这些瞬时分化的细胞显示出与RHO GTPase功能和病毒过程相关的途径的富集，表明SARS-CoV-2的易感性增加。 *注，本文为预印本论文手稿，是未经同行评审的初步报告，其观点仅供科研同行交流，并不是结论性内容，请使用者谨慎使用. 4.附件： 原文链接：https://www.biorxiv.org/content/10.1101/2020.03.13.991455v1
    150浏览量
    原文链接

  • 《BioRxiv，4月25日，Expression of ACE2 and TMPRSS2, the SARS2-CoV-2 receptor and co-receptor, in prostate epithelial cells》
    • 来源专题：COVID-19科研动态监测
    • 编译者：zhangmin
    • 发布时间：2020-04-26
    • Expression of ACE2 and TMPRSS2, the SARS2-CoV-2 receptor and co-receptor, in prostate epithelial cells View ORCID ProfileHanbing Song, Bobak Seddighzadeh, View ORCID ProfileMatthew R Cooperberg, View ORCID ProfileFranklin W Huang doi: https://doi.org/10.1101/2020.04.24.056259 Abstract The COVID-19 pandemic has spread across more than 200 countries and resulted in over 170,000 deaths. For unclear reasons, higher mortality rates from COVID-19 have been reported in men compared to women. While the SARS-CoV-2 receptor ACE2 and co-receptor TMPRSS2 have been detected in lung and other tissues, it is not clear what sex differences may exist. We analyzed a publicly-available normal human prostate single-cell RNA sequencing dataset and found TMPRSS2 and ACE2 co-expressing cells in epithelial cells, with a higher proportion in club and hillock cells. Then we investigated datasets of lung epithelial cells and also found club cells co-expressing TMPRSS2 and ACE2. A comparison of ACE2 expression in lung tissue between males and females showed higher expression in males and a larger proportion of ACE2+ cells in male type II pneumocytes, with preliminary evidence that type II pneumocytes of all lung epithelial cell types showed the highest expression of ACE2. These results raise the possibility that sex differences in ACE2 expression and the presence of double-positive cells in the prostate may contribute to the observed disparities of COVID-19. *注，本文为预印本论文手稿，是未经同行评审的初步报告，其观点仅供科研同行交流，并不是结论性内容，请使用者谨慎使用.
    161浏览量
    原文链接