登录
 机构网站
  1. 首页
  2. 情报产品
  3. 快讯详情

《BioRxiv，3月17日，Characterization of the SARS-CoV-2 Spike in an Early Prefusion Conformation》

  • 来源专题：COVID-19科研动态监测
  • 编译者： zhangmin
  • 发布时间：2020-03-18

  • Characterization of the SARS-CoV-2 Spike in an Early Prefusion Conformation

    Shaowei Li

    doi: https://doi.org/10.1101/2020.03.16.994152

    Abstract

    Pandemic coronavirus disease 2019 (COVID-19) is caused by the emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), for which there are no efficacious vaccines or therapeutics that are urgently needed. We expressed three versions of spike (S) proteins—receptor binding domain (RBD), S1 subunit and S ectodomain—in insect cells. RBD appears monomer in solutions, whereas S1 and S associate into homotrimer with substantial glycosylation. The three proteins confer excellent antigenicity with six convalescent COVID-19 patient sera. Cryo-electron microscopy (cryo-EM) analyses indicate that the SARS-CoV-2 S trimer dominate in a unique conformation distinguished from the classic prefusion conformation of coronaviruses by the upper S1 region at lower position ~15 Å proximal to viral membrane.

    *注，本文为预印本论文手稿，是未经同行评审的初步报告，其观点仅供科研同行交流，并不是结论性内容，请使用者谨慎使用.

  • 原文来源：https://www.biorxiv.org/content/10.1101/2020.03.16.994152v1
56浏览量
原文链接
相关报告

  • 《BioRxiv，3月7日，Direct RNA sequencing and early evolution of SARS-CoV-2》
    • 来源专题：COVID-19科研动态监测
    • 编译者：zhangmin
    • 发布时间：2020-03-08
    • Direct RNA sequencing and early evolution of SARS-CoV-2 George Taiaroa, Daniel Rawlinson, Leo Featherstone, Miranda Pitt, Leon Caly, Julian Druce, Damian Purcell, Leigh Harty, Thomas Tran, Jason Roberts, Mike Catton, Deborah Williamson, Lachlan Coin, Sebastian Duchene doi: https://doi.org/10.1101/2020.03.05.976167 Abstract The rapid sharing of sequence information as seen throughout the current SARS-CoV-2 epidemic, represents an inflection point for genomic epidemiology. Here we describe aspects of coronavirus evolutionary genetics revealed from these data, and provide the first direct RNA sequence of SARS-CoV-2, detailing coronaviral subgenome-length mRNA architecture. *注，本文为预印本论文手稿，是未经同行评审的初步报告，其观点仅供科研同行交流，并不是结论性内容，请使用者谨慎使用.
    106浏览量
    原文链接

  • 《BioRxiv，3月17日，RBD mutations from circulating SARS-CoV-2 strains enhance the structure stability and infectivity of the spike protein》
    • 来源专题：COVID-19科研动态监测
    • 编译者：zhangmin
    • 发布时间：2020-03-18
    • RBD mutations from circulating SARS-CoV-2 strains enhance the structure stability and infectivity of the spike protein Junxian Ou, Zhonghua Zhou, Jing Zhang, Wendong Lan, Shan Zhao, Jianguo Wu, Donald Seto, Gong Zhang, Qiwei Zhang doi: https://doi.org/10.1101/2020.03.15.991844 Abstract A novel zoonotic coronavirus SARS-CoV-2 is associated with the current global pandemic of Coronavirus Disease 2019 (COVID-19). Bats and pangolins are suspected as the reservoir and the intermediate host. The receptor binding domain (RBD) of the SARS-CoV-2 S protein plays the key role in the tight binding to human ACE2 for viral entry. In this study, we analyzed the worldwide RBD mutations and found 10 mutants revealed high positive selection pressure during the spread. The equilibrium dissociation constant (KD) of three RBD mutants were two orders of magnitude lower than the prototype Wuhan-Hu-1 strain due to the stabilization of the beta-sheet scaffold of the RBD. *注，本文为预印本论文手稿，是未经同行评审的初步报告，其观点仅供科研同行交流，并不是结论性内容，请使用者谨慎使用.
    163浏览量
    原文链接