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《BioRxiv，4月13日，（第3版更新）SARS-CoV-2 proteome microarray for mapping COVID-19 antibody interactions at amino acid resolution》

  • 来源专题：COVID-19科研动态监测
  • 编译者： zhangmin
  • 发布时间：2020-04-14

  • SARS-CoV-2 proteome microarray for mapping COVID-19 antibody interactions at amino acid resolution

    Hongye Wang, Xin Hou, Xian Wu, Te Liang, Xiaomei Zhang, Dan Wang, Fei Teng, Jiayu Dai, Hu Duan, Shubin Guo, Yongzhe Li, Xiaobo Yu

    doi: https://doi.org/10.1101/2020.03.26.994756

    Abstract

    COVID-19 has quickly become a worldwide pandemic, which has significantly impacted the economy, education, and social interactions. Understanding the humoral antibody response to SARS-CoV-2 proteins may help identify biomarkers that can be used to detect and treat COVID-19 infection. However, no immuno-proteomics platform exists that can perform such proteome-wide analysis. To address this need, we created a SARS-CoV-2 proteome microarray to analyze antibody interactions at amino acid resolution by spotting peptides 15 amino acids long with 5-amino acid offsets representing full-length SARS-CoV-2 proteins. Moreover, the array processing time is short (1.5 hours), the dynamic range is ~2 orders of magnitude, and the lowest limit of detection is 94 pg/mL. Here, the SARS-CoV-2 proteome array reveals that antibodies commercially available for SARS-CoV-1 proteins can also target SARS-CoV-2 proteins.

    *注，本文为预印本论文手稿，是未经同行评审的初步报告，其观点仅供科研同行交流，并不是结论性内容，请使用者谨慎使用.

  • 原文来源：https://www.biorxiv.org/content/10.1101/2020.03.26.994756v3
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