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《BioRxiv，3月14日，High sensitivity detection of SARS-CoV-2 using multiplex PCR and a multiplex-PCR-based metagenomic method》

  • 来源专题：COVID-19科研动态监测
  • 编译者： zhangmin
  • 发布时间：2020-03-15

  • High sensitivity detection of SARS-CoV-2 using multiplex PCR and a multiplex-PCR-based metagenomic method

    Chenyu Li, David Debruyne, Julia Spencer, Vidushi Kapoor, Lily Y. Liu, Bo Zhang, Lucie Lee, Rounak Feigelman, Grayson Burdon, Jeffrey Liu, Alejandra Oliva, Adam Borcherding, Jian Xu, Alexander E. Urban, Guoying Liu, Zhitong Liu

    doi: https://doi.org/10.1101/2020.03.12.988246

    Abstract

    Many detection methods have been used or reported for the diagnosis and/or surveillance of SARS-CoV-2. Among them, reverse transcription polymerase chain reaction (RT-PCR) is the most sensitive, claiming detection of about 5 copies of viruses. However, it has been reported that only 47-59% of the positive cases were identified by RT-PCR, probably due to loss or degradation of virus RNA in the sampling process, or even mutation of the virus genome. Therefore, developing highly sensitive methods is imperative to ensure robust detection capabilities.

    *注，本文为预印本论文手稿，是未经同行评审的初步报告，其观点仅供科研同行交流，并不是结论性内容，请使用者谨慎使用.

  • 原文来源：https://www.biorxiv.org/content/10.1101/2020.03.12.988246v1
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  • 《3月14日_使用多重PCR和基于多重PCR的宏基因组学方法可高灵敏检测SARS-CoV-2》
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    • 编译者：xuwenwhlib
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