登录
 机构网站
  1. 首页
  2. 情报产品
  3. 快讯详情

《MedRxiv，6月7日，First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan》

  • 来源专题：COVID-19科研动态监测
  • 编译者： zhangmin
  • 发布时间：2020-06-08

  • First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan

    View ORCID ProfileEiji Haramoto, Bikash Malla, View ORCID ProfileOcean Thakali, View ORCID ProfileMasaaki Kitajima

    doi: https://doi.org/10.1101/2020.06.04.20122747

    Abstract

    Wastewater-based epidemiology can be a powerful tool to understand the actual incidence of coronavirus disease 2019 (COVID-19) in a community because severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of COVID-19, can be shed in the feces of infected individuals regardless of their symptoms. The present study aimed to assess the presence of SARS-CoV-2 RNA in wastewater and river water in Yamanashi Prefecture, Japan, using four quantitative and two nested PCR assays. Influent and secondary-treated (before chlorination) wastewater samples and river water samples were collected five times from a wastewater treatment plant and three times from a river, respectively, between March 17 and May 7, 2020. The wastewater and river water samples (200-5,000 mL) were processed by using two different methods: the electronegative membrane-vortex (EMV) method and the membrane adsorption-direct RNA extraction method. Based on the observed concentrations of indigenous pepper mild mottle virus RNA, the EMV method was found superior to the membrane adsorption-direct RNA extraction method.

    *注，本文为预印本论文手稿，是未经同行评审的初步报告，其观点仅供科研同行交流，并不是结论性内容，请使用者谨慎使用.

  • 原文来源：https://www.medrxiv.org/content/10.1101/2020.06.04.20122747v1
6浏览量
原文链接
相关报告

  • 《STTE，5月23日，First detection of SARS-CoV-2 in untreated wastewaters in Italy》
    • 来源专题：COVID-19科研动态监测
    • 编译者：zhangmin
    • 发布时间：2020-05-26
    • First detection of SARS-CoV-2 in untreated wastewaters in Italy Author links open overlay panelGiuseppinaLa RosaaMarcelloIaconelliaPamelaManciniaGiusyBonanno FerraroaCarolinaVeneriaLuciaBonadonnaaLucaLucentiniaElisabettaSuffredinib Show more https://doi.org/10.1016/j.scitotenv.2020.139652 Abstract Several studies have demonstrated the advantages of environmental surveillance through the monitoring of sewage for the assessment of viruses circulating in a given community (wastewater-based epidemiology, WBE). During the COVID-19 public health emergency, many reports have described the presence of SARS-CoV-2 RNA in stools from COVID-19 patients, and a few studies reported the occurrence of SARS-CoV-2 in wastewaters worldwide. Italy is among the world's worst-affected countries in the COVID-19 pandemic, but so far there are no studies assessing the presence of SARS-CoV-2 in Italian wastewaters. To this aim, twelve influent sewage samples, collected between February and April 2020 from Wastewater Treatment Plants in Milan and Rome, were tested adapting, for concentration, the standard WHO procedure for Poliovirus surveillance. Molecular analysis was undertaken with three nested protocols, including a newly designed SARS-CoV-2 specific primer set.
    95浏览量
    原文链接

  • 《Nature，9月18日，Measurement of SARS-CoV-2 RNA in wastewater tracks community infection dynamics》
    • 来源专题：COVID-19科研动态监测
    • 编译者：zhangmin
    • 发布时间：2020-10-13
    • Measurement of SARS-CoV-2 RNA in wastewater tracks community infection dynamics Jordan Peccia, Alessandro Zulli, Doug E. Brackney, Nathan D. Grubaugh, Edward H. Kaplan, Arnau Casanovas-Massana, Albert I. Ko, Amyn A. Malik, Dennis Wang, Mike Wang, Joshua L. Warren, Daniel M. Weinberger, Wyatt Arnold & Saad B. Omer Nature Biotechnology volume 38, pages1164–1167(2020) Abstract We measured severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA concentrations in primary sewage sludge in the New Haven, Connecticut, USA, metropolitan area during the Coronavirus Disease 2019 (COVID-19) outbreak in Spring 2020. SARS-CoV-2 RNA was detected throughout the more than 10-week study and, when adjusted for time lags, tracked the rise and fall of cases seen in SARS-CoV-2 clinical test results and local COVID-19 hospital admissions. Relative to these indicators, SARS-CoV-2 RNA concentrations in sludge were 0–2 d ahead of SARS-CoV-2 positive test results by date of specimen collection, 0–2 d ahead of the percentage of positive tests by date of specimen collection, 1–4 d ahead of local hospital admissions and 6–8 d ahead of SARS-CoV-2 positive test results by reporting date. Our data show the utility of viral RNA monitoring in municipal wastewater for SARS-CoV-2 infection surveillance at a population-wide level. In communities facing a delay between specimen collection and the reporting of test results, immediate wastewater results can provide considerable advance notice of infection dynamics.
    104浏览量
    原文链接