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《CID，3月28日，Quantitative Detection and Viral Load Analysis of SARS-CoV-2 in Infected Patients》

  • 来源专题：COVID-19科研动态监测
  • 编译者： zhangmin
  • 发布时间：2020-03-29

  • Quantitative Detection and Viral Load Analysis of SARS-CoV-2 in Infected Patients

    Fengting Yu, Liting Yan, Nan Wang, Siyuan Yang, Linghang Wang, Yunxia Tang, Guiju Gao, Sa Wang, Chengjie Ma, Ruming Xie ... Show more Author Notes

    Clinical Infectious Diseases, ciaa345, https://doi.org/10.1093/cid/ciaa345

    Published: 28 March 2020

    Abstract

    Background

    Coronavirus disease 2019 (COVID-19) has become a public health emergency. The widely used reverse transcription PCR (RT-PCR) method has limitations for clinical diagnosis and treatment.

    Methods

    A total of 323 samples from 76 COVID-19 confirmed patients were analyzed by droplet digital PCR (ddPCR) and RT-PCR based two target genes (ORF1ab and N). Nasal swabs, throat swabs, sputum, blood, and urine were collected. Clinical and imaging data were obtained for clinical staging.

  • 原文来源：https://academic.oup.com/cid/advance-article/doi/10.1093/cid/ciaa345/5812997?searchresult=1
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