To assess the therapeutic effect of management for lymphatic malformation (LM) in infants. This retrospective study recruited clinical data of 996 patients with LM from June 2004 to July 2015 in our center. All patients were diagnosed as LM after ultrasound, CT or MR scan. All patients were divided into Group 1 (427 patients, treated by endoscopic LM partial resection combined with cautery and postoperative intratumoral negative pressure and absolute ethyl alcohol),Group 2 (239 patients, treated by combined pinyangmycin and dexamethasone injection),Group 3 (330 patients, treated by surgical resection only).The clinical effects were observed in three groups, and therapeutic effect differences in gender, age, maximum diameter, location, range, histological typing, lymph property and treatments were analysed in cervicofacial LM. Group 1:333 patients were cured (78.0％),Group 2:165 patients were cured (69.0％),Group 3:238 patients were cured (72.1％).The difference in cure rate between Group 1 and Group 2 or between
To detect the FcγRⅡB content of monocytes and B cells,the concentration of soluble FcγRⅡB( s FcγRⅡB) and its antibodies in the peripheral blood of patients with lung squamous cell carcinoma( LSCC) before and after chemotherapy. The expression and localization of FcγR Ⅱ B on monocytes and B cells were detected by immunofluorescence technique; the expression of FcγR Ⅱ B mRNA was examined by quantitative real-time PCR; Western blotting was performed to detect the expression of FcγRⅡB protein; the serum concentrations of s FcγRⅡB,s FcγRⅡB-Ig G complex,anti-FcγRⅡB autoantibody were determined by ELISA. The expression of FcγRⅡB on monocytes and B cells,the total s FcγRⅡB and anti-FcγRⅡB antibody content in the sera of LSCC patients were lower than those in healthy volunteers. Chemotherapy enhanced the expression of FcγRⅡB both on the detected cell surface and in the peripheral blood of patients with LSCC,and increased the serum concentration of anti-FcγR Ⅱ B autoantibodies as well. In addition,the concentration of
To preliminarily explore the effects of human dermis derived mesenchymal stem cells (hDMSCs) on expressions of α-smooth muscle actin (α-SMA) and decorin (DCN) in hypertrophic scars fibroblasts (HSFB) at different periods,and to explore the feasibility of MSCs in prevention and treatment of HSFBs. hDMSCs were cultured with mechanical method combined with enzyme digestion.The cells of the third generation which were well grown were taken,and flow cytometry (FCM) was used to detect CD molecules in hDMSCs.Immunocytochemistry was used to detect cytokeratin 19 (CK19) and vimentin and identify the separated cells.The cells were differentiated into lipoblasts,chondroblasts and osteoblasts.According to the formation course of hypertrophic scar,the scar specimens were divided into 6-month,l-year,and 2-year group with three cases in each group.HSFBs from different groups were co-cultured with well-adherent hDMSCs of the third generation in non-contact transwell co-culture system for 21 days. And HSFBs from the correspon
Objective To investigate the clinical effect of individual prefabricated reconstructive titanic plate combined with vascularized iliac bone-muscular flap for reconstruction of mandibular defects. From Aug.2010 to Dec.2014,12 cases with mandibular tumor received preoperative maxillofacial CT scans and 3-dimensional reconstruction. Based on the CT results, mirror imaging technology was used to simulate the reconstruction of the defect at affected side. The individual reconstructive titanium plate was prefabricated on the model. The iliac bone-muscular flap was designed according to the defect shape and size, combined with reconstructive plate. All patients underwent CT scan and oral curved surface tomography postoperatively. The maxillofacial function and appearance were also evaluated. According to the CT scan and curved surface tomography, the 12 iliac bone-muscular flaps survived completely with good bone union and good condyle position. Both the functional and cosmetic results were satisfactory. There was n
To evaluate the effect of hyperbaric oxygen preconditioning on the expression of intercellular adhesion molecule-1 (ICAM-1),vascular cell adhesion molecule-1 (VCAM-1),NF-κB and flap survival rate during Ischemia-Reperfusion Injury in Rat Abdominal Skin Flap. 18 male adult SD rats were divided randomly into sham group (SH),ischemia-reperfusion group (IR) and hyperbaric oxygen preconditioning group (HBO),6 rats in each group. The rats in HBO group received regular hyperbaric oxygen treatment for three days (twice a day with an interval of 12 h) before operation. An abdominal skin flap pedicled with superficial epigastric artery was established. Ischemia lasted for 3 hours in the IR group and HBO group.On the third postoperative day,samples were taken to evaluate the expression of ICAM-1,VCAM-1 and NF-κB by immunohistochemistry staining and Western Blot. In the IR group, the flap survival rate and average blood perfusion were (22.38 ±4.35) ％ and (32.61 ±5.68) PU, while in the HBO group they were (45.34 ±3.15) ％
To explore the feasibility and effect of the lateral popliteal artery perforator flap in repairing the skin and soft tissue defect on upper calf. The position and course of the perforators were confirmed with the color Doppler ultrasound. The flap was designed with the lateral popliteal artery perforator as the pedicle artery to repair the skin and soft tissue defect on upper calf according to the shape and size of the wound. From January 2010 to January 2014,10 patients with traumatic upper calf skin and soft tissue defect were admitted. The wound size ranged from 3 cm ×2 cmto9 cm×6 cm, and the flap size ranged from3 cm×3 cm to 10 cm×8 cm. The wounds at donor sites were closed directly in 4 cases and were covered with split thickness skin graft in 6 cases. All flaps survived completely. Surgical incisions and wounds at donor sites and recipient sites healed primarily. All cases were followed up for 6-15 months (10 months on average) with good flaps color, texture and shape. The lateral popliteal artery perfo
To describe a minimal invasive approach in the Le Fort Ⅲ osteotomy procedure. 33 cases with midfacial hypoplasia were treated by Le Fort Ⅲ osteotomy.Incisions were performed with minimal invasive approach,through lower eyelid,intraoral and upper eyelid crease or medial part of eyebrow instead of coronal incision.After the completion of osteotomy,distraction osteogenesis or orthognathic procedures were followed. The midfacial segments achieved enough advancement without obvious relapse.Bleeding and operative time were reduced compared with coronal approach.No obvious scar was left. The Le Fort Ⅲ osteotomy procedure could be successfully performed through minimal approach with steady results and minimal scars.