AIM To study the activation of CTLs against gastric cancer cells induced by FasL/B7-l (FB-ll)genes modified tumor cells, and to explore whether co-expression of FasL and B7-1 in SGC-7901 tumor cells could initiate an synergistic antitumor effect. METHODS FasL and B7-1 genes were transfected into human SGC-7901 gastric cancer cells with adenovirus vectors. The positive clones were selected by G418. FasL and B7-1 were detected by Flow cytometry and RT-PCR .The abdominal infiltrating lymphocytes and sensitized spleen cells were obtained from the mice who were immunized with SGC-7901/FB-11 or wild type SGC-7901 cells intraperitoneally, and the cytotoxicity of these CTLs against tumor cells was determined by MTT assay. RESULTS Flow cytometry and RT-PCR showed that FasL and B7-1 were highly expressed. FasL + /B7 - ¿SGC-7901 cells (SGC-7901/FB-11) were inoculated subcutaneously in the dorsal skin of C57BL/6 mice and then they decreased their tumorigenicity greatly (p < 0.01). The SGC-7901/FB-11 cell-sensitized mice obtained the protective immune activity against the rechallenge of wild type SGC-7901 cells (p < 0.05). It was showed that the cytotoxicity of CTLs induced by SGC-7901/FB-11 cells against SGC-7901 was significantly higher than that of CTLs activated by wild-type SGC-7901 cells (p < 0.05). CONCLUSION The results suggest that the FasL and B7-1 can effectively promote the activity of CTLs against gastric cancer cells. And FasL/B7-l molecules play an important role in CTL cytotoxicity function as well. After introduced into SGC-7901 cells, Ad-B7-1 shows enhanced therapeutic efficiency for SGC-7901 cells when combined with Ad-FasL.
Background Agenesis of the dorsal pancreas is a very rare congenital pancreatic malformation and is associated with some other diseases. Methods A PubMed search revealed 53 cases of agenesis of the dorsal pancreas. Results In 28 patients with this congenital malformation hyperglycemia was demonstrated, 27 had abdominal pain, 16 had pancreatitis, 14 had an enlarged or prominent pancreatic head visible on computed tomography, and in a few cases, polysplenia, which may occur with various congenital anomalies of visceral organs, was described. Conclusions Difficulties involved in obtaining a firm diagnosis have led to a variety of terms being used to describe this congenital disease. Diagnosis of agenesis of the dorsal pancreas is inconclusive without demonstration of the absence of the dorsal pancreatic duct. Here we describe the embryological development of the pancreas, the so-far known cases of agenesis of the dorsal pancreas with associated medical problems, and the diagnostic measures to find the right conclusions.