To determine the morphologic and physiochemical properties of bovine jugular conduit with valves stabilized by dye-mediated photo-oxidation. Twenty-four bovine jugular conduits with valves were divided into 3 groups and treated with dye-mediated photo-oxidation (Group I), glutaraldehyde (Group II) and untreated group (Group II), respectively. Morphologic and physiochemical properties of the 3 groups, including wall thickness, diameter, tissue water content, heat shrinking temperature, breaching strength, and tissue protein extraction assay were studied. There was no difference in wall thickness, diameter, tissue water content, and heat shrinking temperature between Group I and II ,but there was significant difference between Group I and II. The breaching strength of Group I was higher than that of Group IU (P < 0.05), but lower than that of Group II (P < 0. 05). A decrease in extractable tissue protein was found in Group I and II. The dye-mediated photooxidation can effectively preserve the structure and the
To investigate the effects of compound nylestriol tablet (CNT), containing nylestriol and levonorgestrel with a ratio of 1:0.3) and its components on the osteoporotic rat model induced by retinoic acid (RA) and ovariectomy (OVX). We randomly divided 144 female SD rats (aged 7-month-old) into 12 groups (12 in each). In addition, 120 female SD rats aged 4-month were randomly divided into 10 groups (10 in each). Three dosage levels of CNT (0.039, 0.117 and 0.39 mg/kg body weight, daily), nylestriol (NYL, 0.30, 0.09 and 0.03 mg/kg body weight, daily) and levonorgestrel (LEV, 0.09, 0.027 and 0.009 mg/kg body weight, daily) were designed to prevent the bone loss of the osteoporotic rat model induced by RA and OVX respectively. Serum total calcium (Ca), phosphate (Pi), ALP, triglyceride (TG), total cholesterol (TC), HDL-C, total body BMD, isolated left femoral BMD and femoral Ca, and Pi after ashing were determined. Osteoporotic models could be induced by RA (70 mg/kg body weightdaily given intragastrically for 14 d
To evaluate the efficacy and safety of S (+) - ismer of ibuprofen supporsitory. One hundred and three cases of postopterative pain and 60 cases of fever, altogether 163 patients were randomly divided into 2 groups: S (+) -ismer of ibuprofen supporsitory treatment group (one supporsitory per day for 3 days) and ibuprofen supporsitory treatment group (one supporsitory per day for 3 days). The therapeutic effect was assessed and the side-effects were observed between the 2 groups. After being given medicine in the first 4 hours, pain intensity difference, pain remission degree and the dropped level of fever in S (+) -ismer of ibuprofen were all bigger than those of the ibuprofen supporsitory treatment group. There was no difference in side-effects between the 2 groups. Therapeutic effect of S (+) -ismer of ibuprofen supporsitory is definite, whose action appears more quickly and stronger and it has fewer side-effects than those of ibuprofen supporsitory.
To screen and identify the interactive proteins with connexin 26 (Cx26) by the yeast two hybrid technique. The whole coding region of Cx26 (GJB2) gene was amplified from normal human genomic DNA by polymerase chain reaction (PCR). The "bait" Cx26 was then subcloned into the vector pGBKT7 plasmid of the MatchMaker Gal4 Two-Hybrid System 3 as a target to screen its interactive proteins ("prey") from the human fetal brain eDNA library by the yeast two hybrid technique. The false positive clones were discarded from the preys by one to one yeast two hybrid method between Cx26 and the preys. The DNAs of the preys were sequenced and BLAST analyzed against the GenBank, and also underwent other bioinformatics analysis. The insert of one positive clone contained 145 amino acids residues that was identical to the C-terminal of the neuroendocrine specific protein (NSP) and the open reading frame of the insert was correct. Cx26 is interacted with the C-terminal of NSP. NSP may participate in the process of Cx26 transporta
To investigate the clinical features of patients with cervical spondylosis. Questionnaires were provided and X rays were examined in 1 009 people with different occupations, ages, and sexes. All the patients were diagnozed as cervical spondylosis. Of them, cadres occupied 78.83%, technologists made up 74.21%, and accountants 58.70%; nervous and long-time working people accounted for 59.75%; high and middle pillow-lovers occupied 80.03%. Imaging features: most of the degenerative changes of cervical spine were located between C5-6 (40.79%), C4-5 (26.29%), and C6-7 (18.20%). Patients with vertebral osteophyte were 65.75%, intervertebral space narrow 36.87%, intervertebral foramen narrow 29.19%, and physiological curve change 31.03%. This epidemiologic investigation is important, which can further understand the cause of cervical spondyiosis, and strengthen its prevention and treatment.
To explore the relationship between different left ventricular geometric patterns and ambulatory pulse pressures in essential hypertension patients. Left ventricular geometric patterns were identified according to the results of left ventricular mass index and relative left ventricular thickness assessed by echocardiography in 130 hypertension patients. The average levels of 24-hour pulse pressure, daytime pulse pressure, and nighttime pulse pressure were analyzed by ambulatory blood pressure monitoring. The method of multiple stepwise regression analysis was applied to identify the independent determinants of ambulatory pulse pressures. After controlling or adjusting the effects of agr and the 24-hour mean arterial pressure, the average levels of 24-hour, daytime, and nighttime pulse pressure among different left ventricular geometric patterns were compared. Forty normotensive subjects served as controls. The age and the level of 24-hour mean arterial pressure were the independent determinants of 24-hour, da
To establish an improved radioligand assay of insulin autoantibody (IAA) and to investigate the diagnostic role of IAA in type 1 diabetes (T1DM). 125I-insulin antigens were mixed with sera samples in the Eppendorf tubes. The immunocomplexes were precipitated with protein A-agarose, then washed with TBST buffer and counted the cpm value using liquid scintillation counter. The assay results were expressed by IAA index and compared with those of an international standardized laboratory and a domestic anti-insulin antibody kit. Sera of 32 recent-onset type 1 diabetic patients and 120 healthy controls were screened for IAA and the consistency, sensitivity, specificity and the diagnostic value of IAA were evaluated. (1) The intra-assay coefficient of variation (CV) was 5.8% - 8.3%, and the inter-assay CV 7.0% - 11.0%. The IAA indices of 41 samples tested in our laboratory and in an international standardized laboratory were strongly positively related (r = 0. 783, P < 0. 01) (100.0% concordance). Arrayed according
To research the biomechanic property of 4 tendon grafts. The biomechanic properties of profound hypothermua froze tendon allogeneic grafts, profound hypothermua froze and dried allogeneic tendon grafts, autologous tendon grafts and allogeneic tendon grafts without intervention were tested. The biomechanic property of allogeneic tendon grafts with profound hypothermua freezing and profound hypothermua freezing and dying was better than that of allogeneic tendon grafts without intervention (P 0.05). Allogeneic tendon grafts with profound hypothermua freezing and profound hypothermua freezing and drying, have the same biomechanic property as dried autologous tendon grafts and can be used in clinical practice.
To compare coronary angiography (CAG) and intravascular ultrasound (IVUS). One hundred and twenty-five patients with typical angina who underwent CAG and IVUS were divided into stable angina group (Group SA) and unstable angina group (Group UA). Angiographic feature and ultrasound imaging were compared the two groups. Coronary angiography showed no significant differences in the diseased artery and the degree of stenosis between the 2 groups. Intravascular ultrasound revealed that there were significantly more soft lesions in group UA than in Group SA, and that fibrous and calcific lesions were more prevalent in Group SA. Spontaneous dissection and thrombus were more frequently found in Group UA than in group SA. IVUS is superior to CAG for studying characteristics of coronary atherosclerotic plaque. IVUS is more important than CAG in selecting indications of coronary artery interventional operation.
To evaluate the function and effect of Gamma knife radiosurgery (GKR) in controlling the tumor growth and improving endocrinological abnormality of pituitary adenomas. Two hundred and twenty-eight cases of pituitary adenoma were treated with Leksell Gamma knife. 1.0 Tesla MRI and Gamma-Plan system were used to orientate the tumor. The margin dose was 12 - 35 Gy, mean 21.3 Gy; the center does was 24 - 70 Gy, mean 46.6 Gy (NFA, 31.8 Gy; FA, 50.3 Gy). One hundred and sixteen cases (NFA, 28; FA, 88) were followed up for 4 - 67 months, and the mean was 27.4 months. The growth of tumors in 113 cases (97.4%) was controlled. The tumor volume became smaller in 98 cases (84.5%). Three cases (2.6%) grew larger in tumor size. A significant decrease of excessive hormone production was seen in 89.8% of the patients, and the endocrinological normalization rate was 49.7% (PRL, 47.2%; GH, 57.1%; ACTH, 55.6%). Postradiosurgical complications were seen in 6%. Gamma knife radiosurgery is safe and effective in controlling the tum
To investigate N-methyl-D-aspartate (NMDA)-induced morphological changes in the retina in rats. Thirty-two healthy Sprague-Dawley (SD) rats were randomly divided into 4 groups. Eight rats were in normal control group. The rest 24 rats were equally distributed into 3 experimental groups to receive a single dose of 2 microL intravitreal injection of 3 different concentrations of NMDA (namely 10 nmol, 20 nmol, and 40 nmol per injection ) into the right eyes. The left eyes received 2 microl 0.1 mol PBS used as a self-control. Seven days after the NMDA injection, all rats were killed and the eyes were enucleated. The extent of NMDA-induced neurotoxicity in the retina was quantified by the thickness of the inner plexiform layer (IPL) of the retina at 1.0 - 1.5 mm from the margin of the optic disc of 6 rats in each group. The retinas and optic nerves from the other 2 rats were processed for histopathologic investigation under electronic microscope and light microscope. The thickness of IPL was decreased. There was s
To explore the possibility of bridging the sciatic nerve defects with the composite nerve-muscle (NM) autografts wrapped with human amnion matrix membrane (HAMM) in rats. Fifty-four Wistar rats were divided randomly into 3 groups (n = 18), and about 10 mm of right sciatic nerve defects were bridged with the composite NM autografts, the nerve autografts and the denatured skeletal muscle autografts respectively (Group A, B, and C), and all grafts were wrapped with HAMM. After the operation, the regenerated nerves were assessed by Fast blue retrograde tracing, neurofilament (NF) immnohistochemical staining, regenerated axons counting, measuring the diameter and myelin thickness of the regenerated axons, and quantifying the wet weight of tibialis anterior muscle. In Group A and B, there were more fluorescent-labeled cells in dorsal root ganglion and spinal cord than in Group C. The regenerated NFs were sparse and disordered in Group C, but dense and regular in Group A and B. Group A and Group B showed results sup
To analyze the changes and clinical significance of IL-6 and sICAM-1, and to explore their pathologic mechanism in the brain ischemia. Thirty-two patients at Xiangya Hospital who experienced infarcts that occurred within the first 3 days were consecutively selected into the study, and 30 healthy subjects were selected as controls. The serum level of IL-6 and sICAM-1 was measured by radioimmunoassy and enzyme linked immunosorbent assay. Eleven out of the 32 patients that experienced stroke within 24 hours were observed on the 1st, 3rd, and 6th day. The subsequent volume of brain lesion as a consequence of stroke was measured by CT within 48 - 72 hours after the onset. Both the serum levels of IL-6 and sICAM-1 were significantly higher in patients within the first 3 days after the onset than those of the controls [(352. 1 +/- 31.7) pg/ml vs. (135.4 +/- 18.3) pg/ml, and (363.6 +/- 48.4) ng/ml vs. (227.2 +/- 30.1) ng/ml, P < 0.01]. The levels of IL-6 at the 6th day [(308.3 +/- 26.8) pg/ml] was significantly lower
To investigate the dynamic changes of matrix metalloproteinase-2 (MMP-2) mRNA expression in the posterior sclera of chick form-deprivation myopia (FDM) and its possible molecular mechanism. Fifty white 1-day-old leghorn chicks were divided randomly and equally into 5 groups. The right eye of each chick was covered with a plastic goggle at 4, 7, 14, 21, and 30 postborn days respectively to induce FDM, and the left eye served as a self-control. Meanwhile, normal age-matched chicks were provided as negative control speciments for each group. Removing the goggle at every experiment point, refractive status and axial length were determined with streak retinoscopy (without cycloplegia) and A-scan ultra-sonography under topical anaesthesia, respectively. Both eyes were collected after the chicks were killed. The total RNA in the posterior sclera was extracted traditionally using TRIZOL reagent, and then the expression levels of MMP-2 messenger RNA were analyzed by one step reverse transcriptiontase-polymerase chain
To establish a simple, rapid and precise method to determine platelet serotonin. Serotonin (5-hydroxytryptamine; 5-HT) was determined in platelet pellet after simple deproteinization, following ion-pair high performance liquid chromatography (HPLC) on reverse-phase column: Nova-pak C18 column (150 mm x 4.6 mm, 4 microm), serotonin and internal standard (N-methylserotonin) were detected by fluorometry. The intra-day CV was 2.29% and inter-day CV was 3.88%. The mean recovery of serotonin in platelet was 97.5%, the linear range was 20 - 20000 nmol/ L, and the detection limit was 1 nmol/L. 5-hydroxyindole acetic acid (5-HIAA), Trp, Phe and Tyr had no interference with the chromatographic condition used in this trial. The metod described here is simple, convenient, rapid, sensitive, and accurate for clinical and scientific research.
To observe the influence of bizhongxiao decotion (BZXD) on the plasma TNF-alpha and IL-1beta in rats with C II-induced arthritis (CIA) and to explore the mechanism of BZXD in the treatment of rheumatoid arthritis. We divided 75 rats into 4 groups randomly. The rat experimented arthritis model was established by subcutaneous injection with collagen II. The plasma TNF-alpha and IL-1beta levels were detected with the radio-immunity assay at different time spots. The incidence of arthritis in the rats immunized with C II was approximately 88%. The plasma TNF-alpha and IL-1beta levels of the model group, BZXD group and methotrexate (MTX) group were notably higher than those of the normal group (P < 0.05). The plasma TNF-alpha and IL-1beta levels of the model group were higher than those of the MTX control group and BZXD treatment group at different time spots (P < 0. 01). The plasma TNF-alpha and IL-1beta levels rose step by step, but those of the BZXD group and MTX group decreased gradually. Moreover, the plasma
To explore the predictive value and influence of islet cell antibody (ICA) and glutamic acid decarboxylase antibody (GAD-Ab) for beta cell function in latent autoimmune diabetes in adults (LADA) patients. Fifty-six patients with initially diagnosed type 2 diabetes (including 10 cases of GAD-Ab-positive alone, 14 ICA-positive alone, 7 GAD-Ab and ICA-positive and 25 GAD-Ab and ICA negative) were followed up every 6 months (except the 2nd year) until the 5th year. Their fasting and postprandial C-peptide and glycemic control were measured. GAD-Ab was determined by radioimmunoprecipitation assay and ICA by ELISA kit. Decreased fasting C-peptide was found in patients with GAD-Ab alone and patients with GAD-Ab and ICA in the 2.5th year and to the end of the follow-up. The percentage of patients whose C-peptide decreased 50% or more compared with the baseline in the above 2 groups reached 60.0% in the 3nd year and 71.4% in the 3.5th year, respectively. No changes of the above parameters were found in ICA-positive al