To investigate the effects of rosiglitazone on serum leptin and insulin resistance (IR) in patients with Type 2 diabetes and to determine the correlation between IR and serum leptin. Thirty-nine Type 2 diabetic patients were given rosiglitazone (4 mg/d) for 12 weeks. The changes of height,weight, fasting plasm glucose (FPG), fasting serum insulin (FINS), serum leptin, hepatic and renal function and serum lipid were examined at the baseline and 12 weeks after the therapy. We used the HOMA model to calculate HOMA-IR as an index of insulin resistance. We also calculated the insulin sensitivity index (ISI). The correlation among the above detected markers was analyzed using multiple regression analysis and partial correlation analysis, respectively. The serum leptin level, FINS, and HOMA-IR were higher (P < 0.01), but ISI was lower in the diabetic patients than those of the normal controls before the treatment. BMI, hepatic function, renal function, and serum lipid level did not significantly change, while FPG, F
To detect the expression of MMP-2, MMP-9 and TIMP-2 in pituitary tumors and to explore the relationship among the expressions of MMP-2, MMP-9, TIMP-2 and invasiveness of pituitary adenomas. The expressions of MMP-2, MMP-9 and TIMP-2 were determined in invasive pituitary adenomas (n=20) and non-invasive pituitary adenomas (n=10) by using immunohistochemical method and comparative reverse transcription-polymerase chain reaction (RT-PCR). The correlation between expressions of MMP-2, MMP-9, TIMP-2 and tumor invasiveness were studied. Compared with the non-invasive pituitary, expressions of MMP-9 significantly increased (P < 0.01). TIMP-2 expression decreased in invasive pituitary adenomas (P < 0.01). There was a reverse correlation between MMP-2 and TIMP-2 at mRNA level. Invasive pituitary tumors are related to the high expression of MMPs and low expression of TIMP-2, suggesting that the expression of MMA-9 and TIMP-2 can be used as an index of evaluating invasive pituitary tumors.
To detect the release of glycosylphosphatidylinositol (GPI) anchored CD55 and CD59, and the effect of complement dependent cytotoxicity on K562 cell with expression of GPI-PLD by glucose or insulin. CD55 and CD59 were detected by Western blotting; GPI-PLD activities were analyzed quantitatively; and complement dependent cytotoxicity (CDC) effects were observed by staining of trypan blue. After being induced by insulin or glucose for 24 h and 48 h, GPI-PLD activities and rate of CDC killing in the insulin, insulin + glucose groups significantly increased. At 24 h after the inducement, CD55 was found in the membrane proteins in the control, glucose and insulin groups and CD59 in membrane proteins in the control, glucose group, and medium supernatants of insulin, glucose + insulin groups. Both CD55 and CD59 were found in membrane proteins in control group, and medium supernatants of glucose, insulin, glucose + insulin group at 48 h after the inducement. Treatment with insulin resulted in the obvious increase of
To investigate the influence of EGCG on H2O2-induced gene expression of manganese superoxide dismutase (MnSOD or SOD2) in cultured spiral ganglion cells (SGCs) in vitro. SGCs were cultured in vitro, and H2O2 and/or EGCG in different concentrations were used. Semi-quantitative RT-PCR was applied to observe MnSOD gene expression in SGCs after H2O2 and EGCG treatment. The expression of MnSOD gene was up-regulated with the increase of the concentration of H2O2 in cultured SGCs, and MnSOD gene expression was significantly up-regulated at a dose of H2O2 > or =100 micromol/L. However, this up-regulation was suppressed after simultaneously treated with 100 microg/ml EGCG. EGCG suppresses H2O2-induced up-regulation of MnSOD gene expression in cultured SGCs by getting rid of oxygen free radicals, reinforcing the activity of antioxidant enzymes such as MnSOD, and protects cultured SGCs from H2O2-induced oxidizing damage.
To explore the role of platelet associated immunoglobulin (PAIg) in the mechanism of thrombocytopenia associated with chronic liver disease. The levels of PAIg, PAIgG, PAIgA, and PAIgM were detected in 48 viral hepatitis patients with thrombocytopenia, 36 viral hepatitis patients with normal platelet count and 20 healthy subjects by flow cytometry. PAIg and PAIgG levels (34.54 +/- 14.54%, 28.98 +/- 14.77%, respectively) in the 48 viral hepatitis with thrombocytopenia were significantly higher than those [(21.12 +/- 10.88)%, (16.60 +/- 7.83)%, respectively, P < 0.001)] in the 36 viral hepatitis with normal platelet count and those [(21.15 +/- 16.15) %, (14.89 +/- 9.57)% , respectively, P < 0.01)] in the 20 healthy controls. An inverse correlation was found between the platelet count and PAIg levels (r = -0.446, P < 0.01), and an inverse correlation was also observed between the platelet count and PAIgG levels (r = -0.462, P < 0.01). Autoimmune mechanism mediated by PAIg may play an important role in thrombocyt
To investigate the influence of vector plasmid containing the shRNA of TGFbeta1 on the TGFbeta1 expression in human peritoneal mesotnelial cells. the TGFbeta1 expression in human peritoneal mesothelial cells (HPMCs) by a vector plasmid containing the shRNA of TGFbeta1. The 2 selected fragments of coding sequence contained 21 nts, starting with ggcc. Two pairs of oligos were designed for these two fragments. After annealing double stranded DNA formed, they were ligated to plasmid pcDU6 (pcDNA3.1(-) with U6 promoter) separately. The inverted motif contained 6 spacer and 4 Ts, which made it possible to form short hairpin RNA. Human peritoneal mesothelial cells were isolated from human omental specimens by trypsin disaggregation to establish a stable culture model. Plasmid pcDU6 mediating the expression of TGFbeta1 and plasmid pcDNA3.1(-) mediating the expression of antisense TGFbeta1 RNA were transfected into the third passage HPMCs stimulated by 4. 25% D-glucose and lipopolysaccharide (LPS, 10 microg/ml) by lip
To investigate the renal expression of monocyte chemoattractant protein-1 (MCP-1) in rat with unilateral ureteral obstruction (UUO) and its association with macrophages infiltration in renal interstitium. Sixty female Sprague-Dawley rats were randomized into UUO and sham-operation (SOR) groups. The kidneys were harversted at 1, 4, 7, 10 and 14 d after the operation. Immunohistochemistry was used on the renal tissue for MCP-1 and ED-1 which was a marker of macrophage. Histological changes were also observed by HE and Masson staining. Compared with the SOR group, there was a significant increase in MCP-1, ED-1 expression in the UUO group (P < 0.05). The expression intensity of MCP-1 was correlated with the interstitial macrophages accumulation and interstitial fibrosis (r = 0.865, 0.928, 0.858, 0.893, and 0. 873; r = 0.856, 0.896, 0.686, 0.820, and 0.867, respectively; P < 0.05). Interstitial macrophage infiltration was correlated with the grade of interstitial fibrosis too (r = 0.942, 0.898, 0.920, 0.914, and
To observe whether severe combined immunodeficiency disease (SCID) mice can reconstitute human cell immune system by adoptive transferring of human peripheral blood CD4+ T-lymphocytes to the peritoneal cavity in SCID mice, and to determine the characteristics and function of SCID mice immune system after the reconstitution. SCID mice were injected mature human CD4+ T-lymphocytes to the peritoneal cavity, accompanied with the stimulation of rIL-2 after the injection. Six weeks after the injection, mice were killed in batch, the form and dimension of liver and spleen were observed. The DNA of human lymphocytes was detected in SCID mouse peripheral blood by PCR amplification. The lymphocytes phenotype of SCID mouse immune organs were assayed with immunohistochemistry. The concentration of human cytokines in SCID mouse blood serum was assayed with ELISA after transplanting xenografts. Intraperitoneal injection of SCID mice with mature human peripheral blood CD4+ T lymphocytes could graft human cell immune system
To investigate the serum soluble CD40 ligand (sCD40L) levels in coronary heart disease and its relationship with MMP-9 and to explore the potential predicting factor for the acute coronary syndrome. Enzyme-linked immunosorbent assay was used to measure the sCD40L and MMP-9 in 79 patients with coronary heart disease. sCD40L level was significantly higher in patients with acute coronary syndrome [(1.96 +/- 0.84) ng/ml and (2.23 +/- 0.99) ng/ml in unstable angina and acute myocardial infarction group, respectively] than in stable coronary heart disease patients [(1.20 +/- 0.76) ng/ml, P < 0.05]. Serum sCD40L was significantly correlated with MMP-9 (r = 0.401 , P < 0.01), and sCD40L level was significantly correlated with TG (r = 0.254, P = 0.039), HDL (r = -0.234, P = 0.031), and HDL-C (r = -0.253, P = 0.024). Serum sCD40L and MMP-9 levels were elevated in acute coronary syndrome, suggesting the possible relation to the pathogenesis of ACS and it may serve as a potential marker of plaque stability.
To investigate the pathway and mechanism of parathyroid hormone (PTH) in regulating bone resorption. We observed the regulative effects of PTH on the expression of osteoprotegerin (OPG) and osteoprotegerin ligand (OPGL) and their related cytokines (M-CSF and TRAIL) in human osteoblasts (HOBs). The expressions of OPG, OPGL, M-CSF, and TRAIL mRNA were determined by RT-PCR. The expressions of OPG and OPGL proteins were measured by Western blotting. PTH down-regulated the expression of OPG but up-regulated the expressions of OPGL, M-CSF and TRAIL in HOBs. PTH can promote the bone resorption by decreasing the expression of OPG, increasing the expressions of some bone-resorbing cytokines such as OPGL, M-CSF, and TRAIL in osteoblasts, and then stimulating the osteoclast differentiation and activity.
To make clear the relationship between the X chromosome abnormality and sydromic deafness through genetic analysis of a pedigree with X-linked syndromic deafness. The chromosome number and structure of the family members were analyzed by the standard and high resolution banding with Giemsa, and fluorescent in situ hybridization. The allelic number of the DNA segment in X chromosome was studied with genetic markers. The 2 probands, their mothers and grandmother with normal phenotype all had X(p22-pter) duplication. The whole X chromosome of both the proband III-3 and his mother could be stained with X chromosome staining probe. The proband III-3 had 2 copies of DXS7108. The abnormal X chromosome occurring in this pedigree of X-linked syndromic deafness derives from partial Xp duplication, which will guide further research to identify the breakpoint of this abnormal chromosome.
To evaluate the efficacy of a 5-day rabeprazole-based triple therapy regimen for helicobacter pylori infection, and to improve the eradication rates of antibiotic-resistant Helicobacter pylori with rabeprazole plus clarithromycin and furazolidone. We selected 95 H. pylori-positive patients with gastric or duodenal ulcers or erosive gastritis and randomized them into 2 groups. The patients were given a 7-day triple standard therapy and 5-day triple standard therapy, consisting of rabeprazole 10 mg twice daily, clarithromycin 500 mg twice daily and furazolidone 100 mg twice daily. H. pylori status was checked by rapid urease test or 14C urea breath test and histology before and 4 weeks after the therapy. The eradication rate of H. pylori for patients under the 5-day treatment vs. 7-day treatment were 77.8% vs. 82% by per protocol and 94.4% vs. 97.6% by intention to treat analysis (no significant comparisons). The ulcer and erosion healing rate was 97.6% vs. 97.8% (no significant comparisons). No major side-effe
To determine the effect of transforming growth factor beta1 (TGFbeta1) antisense RNA on the synthesis and secretion of extracellular matrix in human peritoneal mesothelial cells (HPMC). The recombinant human TGFbeta1 antisense eukaryotic expression vector was transfected into HPMC. Fibronectin (FN) and plasminogen activator inhibitor-1 (PAI-1) were detected by ELISA method. The expression of FN, collagen I (col I) and PAI-1 mRNA were detected by RT-PCR method. The mRNA of FN, Col I,nd PAI-1 in HPMC were repressed by TGFbeta1 antisense RNA 24 hours after the transfection in comparison with the control group, which decreased 17%, 26%, and 9.6%, respectively. The protein of FN and PAI-1 were inhibited 48 hours after the transfection (P < 0.05). Recombinant human TGFbeta1 antisense eukaryotic expression vector can inhibit the synthesis and secretion of extracellular matrix in HPMC which may be effective in gene therapy for peritoneal fibrosis.
To provide some references for defining the Chinese optimal intensity of anticoagulation after mechanical heart valve prostheses replacement. For the 178 patients with carbomedics mechanical prosthetic heart valves, the means of INR were compared between the patients with complications and those without complications at the standard of INR1. 4 - 2.0. Also, the variations of INR were compared among different follow-ups. During the follow-up, 22 hemorrhagic and 1 thromboembolic complication occurred. The total linearized rate of anticoagulation-related hemorrhage was 5.83% pty. The total linearized rate thromboembolism was 0.26% pty. The late mortality was 0.79% pty (3 cases ). The final mean INR was 1.68 +/- 0.38. The final mean oral warfarin dose was 2.34 +/- 0.80 mg. The differences of variations of INR in five periods were significant (F = 5.072, P < 0.05). The mean INR in the first month of follow-up was 1.75 +/- 0.27. For Chinese patients with mechanical prosthetic heart valve, hemorrhage is the principal
To investigate the clinical features of patients with cervical spondylosis. Questionnaires were provided and X rays were examined in 1 009 people with different occupations, ages, and sexes. All the patients were diagnozed as cervical spondylosis. Of them, cadres occupied 78.83%, technologists made up 74.21%, and accountants 58.70%; nervous and long-time working people accounted for 59.75%; high and middle pillow-lovers occupied 80.03%. Imaging features: most of the degenerative changes of cervical spine were located between C5-6 (40.79%), C4-5 (26.29%), and C6-7 (18.20%). Patients with vertebral osteophyte were 65.75%, intervertebral space narrow 36.87%, intervertebral foramen narrow 29.19%, and physiological curve change 31.03%. This epidemiologic investigation is important, which can further understand the cause of cervical spondyiosis, and strengthen its prevention and treatment.
To analyze the clinical and neuroimage characteristics of primary central nervous system lymphoma and explore the methods of treatment. The clinical data of 28 cases of primary central nervous system lymphoma were analyzed retrospectively. All the 28 patients with lyphoma were proved by craniotomy and pathologic study. The survival periods were 5 days to 40 months after the craniotomy. Eighteen patients received radiotherapy after the operation. Sixteen recurrent cases were proved by neuroimage and the minimum recurrent time was the 29th day after the operation. The duration of primary lymphoma in the central nervous system is short and the clinical symptom is serious. The neuroimage of primary lymphoma in the central nervous system has some characteristic changes. The recurrence may occur over a brief time after the operation even though the tumor has been totally removed under the microscope. The majority of lymphomas are sensitive to radiotherapy.
To investigate the effects of compound nylestriol tablet (CNT), containing nylestriol and levonorgestrel with a ratio of 1:0.3) and its components on the osteoporotic rat model induced by retinoic acid (RA) and ovariectomy (OVX). We randomly divided 144 female SD rats (aged 7-month-old) into 12 groups (12 in each). In addition, 120 female SD rats aged 4-month were randomly divided into 10 groups (10 in each). Three dosage levels of CNT (0.039, 0.117 and 0.39 mg/kg body weight, daily), nylestriol (NYL, 0.30, 0.09 and 0.03 mg/kg body weight, daily) and levonorgestrel (LEV, 0.09, 0.027 and 0.009 mg/kg body weight, daily) were designed to prevent the bone loss of the osteoporotic rat model induced by RA and OVX respectively. Serum total calcium (Ca), phosphate (Pi), ALP, triglyceride (TG), total cholesterol (TC), HDL-C, total body BMD, isolated left femoral BMD and femoral Ca, and Pi after ashing were determined. Osteoporotic models could be induced by RA (70 mg/kg body weightdaily given intragastrically for 14 d
To screen and identify the interactive proteins with connexin 26 (Cx26) by the yeast two hybrid technique. The whole coding region of Cx26 (GJB2) gene was amplified from normal human genomic DNA by polymerase chain reaction (PCR). The "bait" Cx26 was then subcloned into the vector pGBKT7 plasmid of the MatchMaker Gal4 Two-Hybrid System 3 as a target to screen its interactive proteins ("prey") from the human fetal brain eDNA library by the yeast two hybrid technique. The false positive clones were discarded from the preys by one to one yeast two hybrid method between Cx26 and the preys. The DNAs of the preys were sequenced and BLAST analyzed against the GenBank, and also underwent other bioinformatics analysis. The insert of one positive clone contained 145 amino acids residues that was identical to the C-terminal of the neuroendocrine specific protein (NSP) and the open reading frame of the insert was correct. Cx26 is interacted with the C-terminal of NSP. NSP may participate in the process of Cx26 transporta