The study area locates at wellblock-28-8 of the centre of Gudao oil field,Bohai Bay Basin. It is structurally southern Gudao drape anticline. The typical crowd-well area of wellblock-28-8 was selected as the main project, and the up segment of Guantao Formation as the study target. The stratigraphic model have been set up to research the sedimentary facies and high resolution sequences stratigraphy, in order to make sure the reservoir characteristic using the basic information, such as the cores, seismic and log. And then, the reservoir attributes could be identified, and the knowledge base of reservoir geology were set up. By making use of all kinds of interpolation and simulation methords of geo-statistics, selecting the suitable stochastic simulation approaches, the structural models could be formed. And then, the reliability and practicability of the models were inspected by means of cross check and fractal analysis. On basis of all the forward steps, gridding could be done, and last 3D- visualization were realized.In this dissertation, delicate comparison and classfication of the sublayers have been done, by combining the quiescent research and dynamic productivity, taking the comparison of sediment cycle-sediment time cells as the main methord, and following many other comparison orders. On the basis of the delicate comparison of sublayers, hole deviation correction and K.B. correction, the mini-structure of the upsurface and subsurace of main reservoir sublayers of Guantao Formation were studyed, which had been dentified as river faces. Three mini-structure in the area were identified: forward direction structure, negative direction structure and sloping direction structure.Following the guide of structural model of river facies, three subfacies and six microfacies were identifyed, as well as three interbedded strata and four barrier beds, by making use of the way of combination of static and dynamic, and the combination of microscope and macroscopic characteristics. And then, the patterns of all kinds of the layers could be known. Making use of the methord - “facies-controlling modelling” in the research, the sedimentary face models were setup by equential denotation simulation, and then, the 3D visualization were found by equential gauss simulation.After finishing the reservoir model, the agreement degree of the model was inspected with actual data, and the results were good. At last, on the base of the model, the reserve in the target area was prodicted.
Marine economical finfish aquaculture formed the fourth wave of Marine Aquaculture in China, acquired obvious economic value and social value, push Chinese marine aquaculture. However, during the development of marine aquaculture, there have some unresolved problems, especially the critical periods during the course of larval culture, still a obstacle hinder the stable and healthy development our Chinese marine aquaculture industries.The key element of the marine finfish aquaculture is fingerling production with good quality and high survival rate. However, due to the abundant biodiversity of fish, we must modify our protocol in order to supply relevant species with the most suitable technology. At last, the data obtained from morphological and histological observation must be used in direction of the practical works.The early life history of three important economical marine finfish in northern China (hybrid flounder, barfin flounder and Japanese parrotfish) were described the microscope observation and histological study, and further explained the emerged reasons, inner mechanism and relevant protocol of critical periods during larval early ontogeny. The concrete results are as below:1. Barfin flounder: as a cold water flatfish, the early developmental characteristics is:① The eggs were devoid of oil globule, and semi-pelagic feature; the asynchronous cleavage advanced, occurred on the third cleavage stage. The size heterogeneity between blastomeres was obvious. It had a long incubation time, under the temperature of 8 ± 0.3℃, and salinity of 33, it took 9 d to hatch a larvae from the fertilized eggs. The different embryonic stage of barfin flounder had varied resistance to temperature and gastrula stage was sensitive to temperature.② It also had a long period from hatching to feeding, under the temperature of 8-10℃, and salinity of 33, the larvae began exogenous feeding on circa 8-9 dph. A dot melanin on the beak was the main feature when the larvae fed. The yolk-sac disappeared on 14 dph. The special black-brown striped bars were seen at the posterior period of post-larvae stage.③ The goblet cells occurred on the posterior region of pharynx cavity and the anterior region of esophagus, and the gastric glands differentiated on 29 dph, metamorphosis began at 30 dph. During the early life stage, abundant acidophilic granules on the rectal epithelium indicated the protein-absorbing ability of the larvae intestine.④ The first lymphoid organ was head kidney, then thymus and spleen, which was different to the most marine teleosts. Besides head kidney and spleen, thymus also formed the MMCs. The MMCs in spleen was most abundant.2. Hybrid flounder: as a hybrid of Japanese flounder and summer flounder-the same family, its early developmental characteristics is:① Under the temperature of 15.4～16.0℃, the embryos of hybrid flounder took 76 h to hatch, before the blastopore closure stage, the embryo first formed the optic vesicle and kuffer’s vesicle, then forming somites. Before hatching, the embryoic tail and nose never touched in egg membrane.② The larvae began to feeding on 4 dph, and the yolk-sac & oil globule disappeared on 5 dph & 10 dph, respectively. The intervals of metamorphosis in hybrid flounder is longer (34-60 dph), and the emergence of the crown-like larval fins was not evident like its maternal flounder, however, the numbers of larval fins were 7-8.③ Through the histological observation, the gastric glands of digestive system appeared later, and the developmental duration of the gastric glands was also slower comparison with its maternal flounder. The increment of the thyroid follicles was not evident, the pale color and fewer numbers. During the early ontogeny of the hybrid flounder, no evident indicated the differentiation of anlage of swim bladder.3. Japanese parrotfish: as a warm water species, lithophilic teleosts, the early developmental feature is:① fertilized eggs:The yolk was segmented, and acts as a feature of fish egg classification.②The newly-hatched larve: The melanin on the larval dorsal finfold in the newly-hatched larvae, moved from to the edge of the larval dorsal finfold, and disappeared on 3 dph. This phenomenon indicated the special ontogenetic characteristics of this species. ③ post-larvae: The thickness of muscular tissue in intestine increased quickly during this period, and the gut evacuation rised abruptly, at the same time, the mortality rate rise up, and we resolved this problem by the means of changing the routine feeding pattern. During the juvenile stage, the jaw teeth of Japanese parrotfish fused into two whole teeth plates, which was a normal feature of Oplegnathidae.④ some secreting cells were found in the thymus during the larval development, which usually were found in some lower finfish, like salmonids. Through our studies, the numbers of secreting cells in rainbow trout was fewer than Japanese parrotfish, and its arrangement was also devoid of orderliness. MMCs was also normally found in the hemopoietic organs-spleen and head kidney, also found in the parenchyma of liver, pancreas and swim bladder, which usually occurred in elasmobranch fishes and few in teleosts