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期刊名称: BMC Developmental Biology
Volume:7    Issue:1        Page:137-137

The first trimester human trophoblast cell line ACH-3P: A novel tool to study autocrine/paracrine regulatory loops of human trophoblast subpopulations - TNF-α stimulates MMP15 expression期刊论文

作者: Hiden Ursula Prutsch Nicole Gauster Martin Weiss Ursula Frank Hans-Georg

页码: 137-137
被引频次: 40
出版者: BMC,BioMed Central Ltd
期刊名称: BMC Developmental Biology
ISSN: 1471-213X
卷期: Volume:7    Issue:1
语言: English
摘要: Background: The trophoblast compartment of the placenta comprises various subpopulations with distinct functions. They interact among each other by secreted signals thus forming autocrine or paracrine regulatory loops. We established a first trimester trophoblast cell line (ACH-3P) by fusion of primary human first trimester trophoblasts (week 12 of gestation) with a human choriocarcinoma cell line (AC1-1). Results: Expression of trophoblast markers (cytokeratin-7, integrins, matrix metalloproteinases), invasion abilities and transcriptome of ACH-3P closely resembled primary trophoblasts. Morphology, cytogenetics and doubling time was similar to the parental AC1-1 cells. The different subpopulations of trophoblasts e. g., villous and extravillous trophoblasts also exist in ACH-3P cells and can be immuno-separated by HLA-G surface expression. HLA-G positive ACH-3P display pseudopodia and a stronger expression of extravillous trophoblast markers. Higher expression of insulin-like growth factor II receptor and human chorionic gonadotropin represents the basis for the known autocrine stimulation of extravillous trophoblasts. Conclusion: We conclude that ACH-3P represent a tool to investigate interaction of syngeneic trophoblast subpopulations. These cells are particularly suited for studies into autocrine and paracrine regulation of various aspects of trophoblast function. As an example a novel effect of TNF-alpha on matrix metalloproteinase 15 in HLA-G positive ACH-3P and explants was found.
相关主题: MIGRATION, INVASION IN-VITRO, MATRIX METALLOPROTEINASES, HUMAN PLACENTA, PLASMINOGEN-ACTIVATOR, PARACRINE REGULATION, NECROSIS-FACTOR-ALPHA, PREECLAMPSIA, DEVELOPMENTAL BIOLOGY, GROWTH-FACTOR, HUMAN CYTOTROPHOBLAST, Immunohistochemistry, Autocrine Communication, Cell Line, Trophoblasts - metabolism, Oligonucleotide Array Sequence Analysis, Paracrine Communication, Cell Separation, Neoplasm Invasiveness, Humans, Chorionic Gonadotropin - secretion, HLA-G Antigens, HLA Antigens - metabolism, Reverse Transcriptase Polymerase Chain Reaction, Pregnancy Trimester, First, Matrix Metalloproteinase 15 - biosynthesis, Choriocarcinoma, Histocompatibility Antigens Class I - metabolism, Pregnancy, Cell Fusion, Tumor Necrosis Factor-alpha - pharmacology, Flow Cytometry, Cell Line, Tumor, Female, Trophoblasts - cytology,






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