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期刊名称: Biology of Reproduction
Volume:80    Issue:6        Page:1136-1145
ISSN:0006-3363

Isolation and Culture of Epithelial Progenitors and Mesenchymal Stem Cells from Human Endometrium期刊论文

作者: Caroline E. Gargett Kjiana E. Schwab Rachel M. Zillwood Hong P.T. Nguyen Di Wu
DOI:10.1095/biolreprod.108.075226

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页码: 1136-1145
被引频次: 252
出版者: Society for the Study of Reproduction,SOC STUDY REPRODUCTION
期刊名称: Biology of Reproduction
ISSN: 0006-3363
卷期: Volume:80    Issue:6
语言: English
摘要: Human endometrium is a highly regenerative tissue undergoing more than 400 cycles of growth, differentiation, and shedding during a woman's reproductive years. Endometrial regeneration is likely mediated by adult stem/progenitor cells. This study investigated key stem cell properties of individual clonogenic epithelial and stromal cells obtained from human endometrium. Single-cell suspensions of endometrial epithelial or stromal cells were obtained from hysterectomy tissues from 15 women experiencing normal menstrual cycles, and were cultured at clonal density (10 cells/cm 2 ) or limiting dilution. The adult stem cell properties—self-renewal, high proliferative potential, and differentiation of single epithelial and stromal cells—were assessed by harvesting individual colonies and undertaking serial clonal culture, serial passaging, and culture in differentiation-induction media, respectively. Lineage differentiation markers were examined by RT-PCR, immunocytochemistry, and flow cytometry. Rare single human endometrial EpCAM + epithelial cells and EpCAM − stromal cells demonstrated self-renewal by serially cloning >3 times and underwent >30 population doublings over 4 mo in culture. Clonally derived epithelial cells differentiated into cytokeratin + gland-like structures in three dimensional culture. Single stromal cells were multipotent, as their progeny differentiated into smooth muscle cells, adipocytes, chondrocytes, and osteoblasts. Stromal clones expressed mesenchymal stem cell (MSC) markers ITGB1 (CD29), CD44, NT5E (CD73), THY1 (CD90), ENG (CD105), PDGFRB (CD140B), MCAM (CD146) but not endothelial or hemopoietic markers PECAM1 (CD31), CD34, PTPRC (CD45). Adult human endometrium contains rare epithelial progenitors and MSCs, likely responsible for its immense regenerative capacity, which may also have critical roles in the development of endometriosis and endometrial cancer. Human endometrium may provide a readily available source of MSCs for cell-based therapies.
相关主题: Mesenchymal stem cell, Adult stem cells, Epithelial progenitor cell, Human endometrium, Endometrial stem cell, Uterus, Female reproductive tract, Differentiation, Clonal assays, mesenchymal stem cell, differentiation, endometrial stem cell, human endometrium, DENTAL-PULP, MARKERS, MOUSE ENDOMETRIUM, IDENTIFICATION, adult stem cells, REPRODUCTIVE BIOLOGY, uterus, HUMAN BONE-MARROW, epithelial progenitor cell, SIDE-POPULATION CELLS, SURFACE, STROMAL CELLS, clonal assays, female reproductive tract, COLONY-FORMING CELLS, ADIPOSE-TISSUE, Adult Stem Cells - physiology, Adult Stem Cells - cytology, Cell Proliferation, Humans, Middle Aged, Mesenchymal Stromal Cells - cytology, Phenotype, Adult, Female, Cell Differentiation, Clone Cells, Endometrium - cytology, Epithelial Cells - cytology, Mesenchymal Stromal Cells - physiology,

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