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期刊名称: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences
Volume:29    Issue:3    
ISSN:1672-7347

Bridging rat sciatic nerve defects with the composite nerve-muscle autografts wrapped with human amnion matrix membrane期刊论文

作者: Duan Xian-Lai Xu Yuan-Zhong Zeng Zhi-Cheng

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期刊名称: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences
ISSN: 1672-7347
卷期: Volume:29    Issue:3
语言: Chinese
摘要: To explore the possibility of bridging the sciatic nerve defects with the composite nerve-muscle (NM) autografts wrapped with human amnion matrix membrane (HAMM) in rats. Fifty-four Wistar rats were divided randomly into 3 groups (n = 18), and about 10 mm of right sciatic nerve defects were bridged with the composite NM autografts, the nerve autografts and the denatured skeletal muscle autografts respectively (Group A, B, and C), and all grafts were wrapped with HAMM. After the operation, the regenerated nerves were assessed by Fast blue retrograde tracing, neurofilament (NF) immnohistochemical staining, regenerated axons counting, measuring the diameter and myelin thickness of the regenerated axons, and quantifying the wet weight of tibialis anterior muscle. In Group A and B, there were more fluorescent-labeled cells in dorsal root ganglion and spinal cord than in Group C. The regenerated NFs were sparse and disordered in Group C, but dense and regular in Group A and B. Group A and Group B showed results sup
相关主题: Sciatic Nerve - injuries, Rats, Wistar, Humans, Muscle, Skeletal - transplantation, Rats, Male, Nerve Regeneration, Random Allocation, Peripheral Nerves - transplantation, Animals, Sciatic Nerve - surgery, Amnion - transplantation, Female,

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