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期刊名称: Biology of Reproduction
Volume:71    Issue:4        Page:1108-1119
ISSN:0006-3363

Oxygen-Regulated Gene Expression in Bovine Blastocysts期刊论文

作者: A.J. Harvey K.L. Kind M. Pantaleon D.T. Armstrong J.G. Thompson
DOI:10.1095/biolreprod.104.028639

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页码: 1108-1119
被引频次: 115
出版者: Society for the Study of Reproduction,SOC STUDY REPRODUCTION
期刊名称: Biology of Reproduction
ISSN: 0006-3363
卷期: Volume:71    Issue:4
语言: English
摘要: Oxygen concentrations used during in vitro embryo culture can influence embryo development, cell numbers, and gene expression. Here we propose that the preimplantation bovine embryo possesses a molecular mechanism for the detection of, and response to, oxygen, mediated by a family of basic helix-loop-helix transcription factors, the hypoxia-inducible factors (HIFs). Day 5 compacting bovine embryos were cultured under different oxygen tensions (2%, 7%, 20%) and the effect on the expression of oxygen-regulated genes, development, and cell number allocation and HIFα protein localization were examined. Bovine in vitro-produced embryos responded to variations in oxygen concentration by altering gene expression. GLUT1 expression was higher following 2% oxygen culture compared with 7% and 20% cultured blastocysts. HIF mRNA expression (HIF1α, HIF2α) was unaltered by oxygen concentration. HIF2α protein was predominantly localized to the nucleus of blastocysts. In contrast, HIF1α protein was undetectable at any oxygen concentration or in the presence of the HIF protein stabilizer desferrioxamine (DFO), despite being detectable in cumulus cells following normal maturation conditions, acute anoxic culture, or in the presence of DFO. Oxygen concentration also significantly altered inner cell mass cell proportions at the blastocyst stage. These results suggest that oxygen can influence gene expression in the bovine embryo during postcompaction development and that these effects may be mediated by HIF2α.
相关主题: Environment, Embryo, Developmental biology, Gene regulation, Early development, developmental biology, FACTOR MESSENGER-RNA, embryo, HUMAN PREIMPLANTATION EMBRYOS, SPLICE VARIANTS, SHEEP CONCEPTUS, IN-VITRO CULTURE, SIGNAL-TRANSDUCTION, DEVELOPMENTALLY IMPORTANT GENES, early development, environment, REPRODUCTIVE BIOLOGY, gene regulation, ENDOTHELIAL-CELLS, TRANSCRIPTIONAL REGULATION, HYPOXIA-INDUCIBLE FACTOR-1, Blastocyst - metabolism, Embryonic Development - physiology, RNA, Messenger - analysis, Blastocyst - cytology, Glucose Transporter Type 1, Vascular Endothelial Growth Factor A - metabolism, Embryo Culture Techniques, Transcription Factors - genetics, Vascular Endothelial Growth Factor A - genetics, Hypoxia-Inducible Factor 1, alpha Subunit, Oxygen - metabolism, Tissue Distribution, Transcription Factors - metabolism, Animals, Cattle, Monosaccharide Transport Proteins - metabolism, Basic Helix-Loop-Helix Transcription Factors, Gene Expression Regulation, Developmental - physiology, Monosaccharide Transport Proteins - genetics, Index Medicus,

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