National Science Library, Chinese Academy of Sciences
  登录 机构网站 ENGLISH

期刊名称: Biology of Reproduction
Volume:91    Issue:5        Page:121-121

Central role of 5'-AMP-activated protein kinase in chicken sperm functions期刊论文

作者: Nguyen Thi Mong Diep Alves Sabine Grasseau Isabelle Métayer-Coustard Sonia Praud Christophe

页码: 121-121
被引频次: 31
出版者: SOC STUDY REPRODUCTION,Society for the Study of Reproduction
期刊名称: Biology of Reproduction
ISSN: 0006-3363
卷期: Volume:91    Issue:5
语言: English
摘要: Avian gametes present specific features related to their internal long-term mode of fertilization. Among other central actors of energetic metabolism control, it has been suspected that 5'-AMP-activated protein kinase (AMPK) influences sperm functions and thus plays a key role in fertilization success. In the present work, we studied AMPK localization and function in chicken sperm incubated in vitro. Effects of the pharmacological AMPK activators (AICAR, metformin) and the AMPK inhibitor compound C were assessed by evaluating AMPKalpha (Thr(172)) phosphorylation (by Western blotting), semen quality (by viability, motility, and ability to perform acrosome reaction), and energetic metabolism indicators (lactate, ATP). Localization of AMPK in subcellular sperm compartments was evaluated by immunocytochemistry. Total AMPK was found in all compartments except for the nucleus, but the phosphorylated form phospho-Thr(172) -AMPK was essentially localized in the flagellum and acrosome. AMPK activators significantly improved AMPK phosphorylation, sperm motility (increased by 40% motile, 90% progressive, and 60% rapid sperm), acrosome reaction and lactate production (increased by 40%) and viability. The AMPK inhibitor significantly reduced AMPK phosphorylation and percentages of motility (decrease by 25%), progressive energy (decrease by 35%), and rapid sperm (decreased by 30%), acrosome reaction, lactate production, and ATP release. The two activators differed in their effect on ATP concentration: AICAR stimulated ATP formation, whereas metformin did not. Our results indicate that AMPK plays a key role in the regulation of chicken sperm functions and metabolism. This action differs from that suggested in mammals, mainly by its crucial involvement in the acrosome reaction process.
相关主题: Signaling pathways, Acrosome reaction, AMPK, Motility, Chicken sperm, 5'-AMP-activated protein kinase, REPRODUCTIVE BIOLOGY, signaling pathways, chicken sperm, motility, 5 '-AMP-activated protein kinase, acrosome reaction, Cell Survival - drug effects, Metformin - pharmacology, Male, Pyrimidines - pharmacology, Spermatozoa - drug effects, Aminoimidazole Carboxamide - pharmacology, Ribonucleotides - pharmacology, AMP-Activated Protein Kinases - physiology, Animals, Aminoimidazole Carboxamide - analogs & derivatives, Chickens, Acrosome Reaction - drug effects, Spermatozoa - physiology, Phosphorylation - drug effects, Pyrazoles - pharmacology, Semen Analysis - veterinary, Spermatozoa - enzymology, Index Medicus, Life Sciences, Other,






在线获取原文 原文传递 详细信息 图书在架状态 图书馆际互借 问图书馆员


图书馆开放时间 图书馆位置 借阅要求 您在使用中发现的任何错误,都可以向我们 【报告错误】,非常感谢!