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期刊名称: Biology of Reproduction
Volume:91    Issue:4        Page:90-90
ISSN:0006-3363

The gametic synapse: RNA transfer to the bovine oocyte期刊论文

作者: Macaulay Angus D Gilbert Isabelle Caballero Julieta Barreto Rodrigo Fournier Eric
DOI:10.1095/biolreprod.114.119867

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页码: 90-90
被引频次: 56
出版者: OXFORD UNIV PRESS INC
期刊名称: Biology of Reproduction
ISSN: 0006-3363
卷期: Volume:91    Issue:4
语言: English
摘要: Even after several decades of quiescent storage in the ovary, the female germ cell is capable of reinitiating transcription to build the reserves that are essential to support early embryonic development. In the current model of mammalian oogenesis, there exists bilateral communication between the gamete and the surrounding cells that is limited to paracrine signaling and direct transfer of small molecules via gap junctions existing at the end of the somatic cells' projections that are in contact with the oolemma. The purpose of this work was to explore the role of cumulus cell projections as a means of conductance of large molecules, including RNA, to the mammalian oocyte. By studying nascent RNA with confocal and transmission electron microscopy in combination with transcript detection, we show that the somatic cells surrounding the fully grown bovine oocyte contribute to the maternal reserves by actively transferring large cargo, including mRNA and long noncoding RNA. This occurrence was further demonstrated by the reconstruction of cumulus-oocyte complexes with transfected cumulus cells transferring a synthetic transcript. We propose selective transfer of transcripts occurs, the delivery of which is supported by a remarkable synapselike vesicular trafficking connection between the cumulus cells and the gamete. This unexpected exogenous contribution to the maternal stores offers a new perspective on the determinants of female fertility.
相关主题: In vitro maturation (IVM), Oocyte development, Intercellular communication, Oocyte maturation, SECRETED FACTORS, SOMATIC-CELLS, TRANSLOCATION, PROTEIN-KINASE, oocyte development, COMPLEXES, DEVELOPMENTAL COMPETENCE, REPRODUCTIVE BIOLOGY, IN-VITRO MATURATION, intercellular communication, GENE-EXPRESSION, CUMULUS CELLS, in vitro maturation (IVM), oocyte maturation, POLYMERASE-II, Animals, Genetically Modified, Oogenesis - physiology, Computational Biology, Gene Expression Regulation, Transcriptome, Cattle - genetics, Cumulus Cells - ultrastructure, Animals, Oocytes - physiology, Female, Cattle - physiology, Cumulus Cells - physiology, RNA - metabolism, Index Medicus,

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