National Science Library, Chinese Academy of Sciences
  登录 机构网站 ENGLISH
您当前的位置是:首页->详细浏览

期刊名称: Biology of Reproduction
Volume:81    Issue:4        Page:784-794
ISSN:0006-3363

Progesterone-Regulated Changes in Endometrial Gene Expression Contribute to Advanced Conceptus Development in Cattle期刊论文

作者: N. Forde F. Carter T. Fair M.A. Crowe A.C.O. Evans
DOI:10.1095/biolreprod.108.074336

服务链接:
页码: 784-794
被引频次: 182
出版者: Society for the Study of Reproduction,SOC STUDY REPRODUCTION
期刊名称: Biology of Reproduction
ISSN: 0006-3363
卷期: Volume:81    Issue:4
语言: English
摘要: The postovulatory rise in circulating progesterone (P4) concentrations is associated with increased pregnancy success in beef and dairy cattle. Our study objective was to determine how elevated P4 alters endometrial gene expression to advance conceptus development. Synchronized heifers were inseminated (Day 0) and randomly assigned to pregnant high P4 or to pregnant normal P4. All high P4 groups received a P4-release intravaginal device on Day 3 after insemination that increased P4 concentrations up to Day 7 ( P < 0.05). Tissue was collected on Day 5, 7, 13, or 16 of pregnancy, and endometrial gene expression was analyzed using the bovine Affymetrix (Santa Clara, CA) microarrays. Microarray analyses demonstrated that the largest number of P4-regulated genes coincided with the day when the P4 profiles were different for the longest period. Genes with the largest fold change increase (such as DGAT2 and MSTN [also known as GDF8 ]) were associated with triglyceride synthesis and glucose transport, which can be utilized as an energy source for the developing embryo. Temporal changes occurred at different stages of early pregnancy, with the greatest difference occurring between well-separated stages of conceptus development. Validation of a number of genes by quantitative real-time PCR indicated that P4 supplementation advances endometrial gene expression by altering the time ( FABP , DGAT2 , and MSTN ) or duration ( CRYGS ) of expression pattern for genes that contribute to the composition of histotroph.
相关主题: Pregnancy, Progesterone, Uterus, Female reproductive tract, Gene regulation, SELECT NUTRIENTS, INTERFERON-TAU, OVINE UTERUS, pregnancy, progesterone, BOVINE ENDOMETRIUM, EMBRYO SURVIVAL, RUMINANTS, DAIRY-COWS, REPRODUCTIVE BIOLOGY, uterus, EARLY-PREGNANCY, gene regulation, GLUCOSE, female reproductive tract, ESTROUS-CYCLE, Gene Expression - drug effects, Oligonucleotide Array Sequence Analysis, Progesterone - administration & dosage, Gene Expression Profiling, Embryonic Development, Pregnancy, Animal - metabolism, Animals, Time Factors, Cattle, Polymerase Chain Reaction, Progesterone - metabolism, Female, Endometrium - metabolism, Index Medicus,

相关文献推荐:

问图书管理员更多图书管理员

学科咨询馆员
学科馆员

电话:
邮件:
问图书馆员

图标说明

在线获取原文 原文传递 详细信息 图书在架状态 图书馆际互借 问图书馆员

常见问题

图书馆开放时间 图书馆位置 借阅要求 您在使用中发现的任何错误,都可以向我们 【报告错误】,非常感谢!

作者信息:×