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期刊名称: Biology of Reproduction
Volume:37    Issue:2        Page:311-316
ISSN:0006-3363

Hypoxanthine causes a 2-cell block in random-bred mouse embryos期刊论文

作者: D Loutradis D John A A Kiessling
DOI:10.1095/biolreprod37.2.311

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页码: 311-316
被引频次: 100
出版者: Society for the Study of Reproduction,OXFORD UNIV PRESS INC
期刊名称: Biology of Reproduction
ISSN: 0006-3363
卷期: Volume:37    Issue:2
语言: English
摘要: Ham's F-10, a chemically defined, complex culture medium, commonly used for in vitro fertilization of human as well as animal oocytes, blocked development at the 2-cell stage of greater than 92% of embryos from random-bred Swiss mice (CD-1), but did not block development of embryos from hybrid-inbred mice (BDF1). In contrast, BWW, a simple, modified Kreb's-Ringer bicarbonate medium, supported development to blastocysts of 85% and 100% of 2-cell embryos from CD1 and BDF1 females, respectively. As little as 15% (v/v) Ham's F-10 added to the BWW blocked the development of the random-bred embryos. Supplementing the BWW with Ham's F-10 components revealed that hypoxanthine (6-30 microM) was responsible for the developmental block to the random-bred embryos. The hypoxanthine block was partially (40%) reversed by adding the chelating agent, ethylenediaminetetraacetic acid. Breeding experiments showed that the hypoxanthine sensitivity of embryos from CD-1 mothers was not affected by the paternal genome.
相关主题: REPRODUCTIVE BIOLOGY, Embryo, Mammalian - drug effects, Animals, Culture Media, Fertilization in Vitro - methods, Female, Hypoxanthine, Mice, Mice, Inbred Strains, Hypoxanthines - pharmacology, Organ Culture Techniques, Index Medicus,

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