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期刊名称: Biology of Reproduction
Volume:91    Issue:5        Page:106-106
ISSN:0006-3363

Putrescine stimulates the mTOR signaling pathway and protein synthesis in porcine trophectoderm cells期刊论文

作者: Kong Xiangfeng Wang Xiaoqiu Yin Yulong Li Xilong Gao Haijun
DOI:10.1095/biolreprod.113.113977

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页码: 106-106
被引频次: 32
出版者: SOC STUDY REPRODUCTION
期刊名称: Biology of Reproduction
ISSN: 0006-3363
卷期: Volume:91    Issue:5
语言: English
摘要: Insufficient placental growth is a major factor contributing to intrauterine growth retardation in mammals. There is growing evidence that putrescine produced from arginine (Arg) and proline via ornithine decarboxylase is a key regulator of angiogenesis, embryogenesis, as well as placental and fetal growth. However, the underlying mechanisms are largely unknown. The present study tested the hypothesis that putrescine stimulates protein synthesis by activating the mechanistic target of rapamycin (mTOR) signaling pathway in porcine trophectoderm cell line 2 cells. The cells were cultured for 2 to 4 days in customized Arg-free Dulbecco modified Eagle Ham medium containing 0, 10, 25, or 50 mu M putrescine or 100 mu M Arg. Cell proliferation, protein synthesis, and degradation, as well as the abundance of total and phosphorylated mTOR, ribosomal protein S6 kinase 1, and eukaryotic initiation factor 4E-binding protein-1 (4EBP1), were determined. Our results indicate that putrescine promotes cell proliferation and protein synthesis in a dose-and time-dependent manner, which was inhibited by difluoro-methylornithine (an inhibitor of ornithine decarboxylase). Moreover, supplementation of culture medium with putrescine increased the abundance of phosphorylated mTOR and its downstream targets, 4EBP1 and p70 S6K1 proteins. Collectively, these findings reveal a novel and important role for putrescine in regulating the mTOR signaling pathway in porcine placental cells. We suggest that dietary supplementation with or intravenous administration of putrescine may provide a new and effective strategy to improve survival and growth of embryos/fetuses in mammals.
相关主题: mTOR, Putrescine, Protein synthesis, Placental cells, PIGS, OVINE CONCEPTUS, FETAL-GROWTH, protein synthesis, placental cells, P70 S6 KINASE, IN-VITRO, REPRODUCTIVE BIOLOGY, POLYAMINES, L-ARGININE, INTRAUTERINE GROWTH RESTRICTION, MELANOMA-CELLS, putrescine, AMINO-ACID NUTRITION, Ectoderm - drug effects, Trophoblasts - metabolism, TOR Serine-Threonine Kinases - metabolism, Cells, Cultured, Eflornithine - pharmacology, Pregnancy, Trophoblasts - drug effects, Up-Regulation - drug effects, Ectoderm - metabolism, Animals, Signal Transduction - drug effects, Swine, Female, Protein Biosynthesis - drug effects, Cell Proliferation - drug effects, Putrescine - pharmacology, Index Medicus,

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