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期刊名称: Biology of Reproduction
Volume:88    Issue:5        Page:112-112

Transgenerational effects of Di-(2-ethylhexyl) phthalate on testicular germ cell associations and spermatogonial stem cells in mice期刊论文

作者: Doyle Timothy J Bowman Jennifer L Windell Veronica L McLean Derek J Kim Kwan Hee

页码: 112-112
被引频次: 88
期刊名称: Biology of Reproduction
ISSN: 0006-3363
卷期: Volume:88    Issue:5
语言: English
摘要: Recent evidence has linked human phthalate exposure to abnormal reproductive and hormonal effects. Phthalates are plasticizers that confer flexibility and transparency to plastics, but they readily contaminate the body and the environment. In this study, timed pregnant CD1 outbred mice were treated with di-(2-ethylhexyl) phthalate (DEHP) from Embryonic Day 7 (E7) to E14. The subsequent generation (F1) offspring were then bred to produce the F2, F3, and F4 offspring, without any further DEHP treatment. This exposure scheme disrupted testicular germ cell association and decreased sperm count and motility in F1 to F4 offspring. By spermatogonial transplantation techniques, the exposure scheme also disrupted spermatogonial stem cell (SSC) function of F3 offspring. The W/W-V recipient testes transplanted with F3 offspring germ cells from the DEHP-treated group had a dramatically lower percentage of donor germ cell-derived spermatogenic recovery in seminiferous tubules when compared to the recipient testes transplanted with CD1 control germ cells. Further characterization showed that the major block of donor germ cell-derived spermatogenesis was before the appearance of undifferentiated spermatogonia. Interestingly, the testes transplanted with the F3 offspring germ cells from the DEHP-treated group, when regenerated, replicated testis morphology similar to that observed in the testes from the F1 to F3 offspring of the DEHP-treated group, suggesting that the germ cell disorganization phenotype originates from the stem cells of F3 offspring. In conclusion, embryonic exposure to DEHP was found to disrupt testicular germ cell organization and SSC function in a transgenerational manner.
相关主题: Testis, Spermatogenesis, DEHP, Transgenerational, Spermatogonial stem cells, Environmental contaminants and toxicants, Male germ cells, DIETHYLSTILBESTROL DES, IN-UTERO EXPOSURE, transgenerational, FETAL TESTIS, DIETHYLHEXYL-PHTHALATE, DI(2-ETHYLHEXYL) PHTHALATE, testis, TESTOSTERONE PRODUCTION, REPRODUCTIVE BIOLOGY, DI(N-BUTYL) PHTHALATE, spermatogonial stem cells, ADULT-ONSET DISEASE, environmental contaminants and toxicants, spermatogenesis, LACTATIONAL EXPOSURE, male germ cells, MONO-(2-ETHYLHEXYL) PHTHALATE, Spermatogonia - drug effects, Sperm Count, Spermatogenesis - drug effects, Prenatal Exposure Delayed Effects, Testis - cytology, Apoptosis - drug effects, Male, Stem Cells - cytology, Plasticizers - toxicity, Spermatozoa - drug effects, Testis - drug effects, Pregnancy, Sperm Motility - drug effects, Animals, Diethylhexyl Phthalate - toxicity, Stem Cells - drug effects, Female, Mice, Spermatozoa - cytology, Spermatogonia - cytology, Seminiferous Tubules - drug effects, Index Medicus,






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