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期刊名称: Biology of Reproduction
Volume:94    Issue:2        Page:38-38
ISSN:0006-3363

Human endometrial exosomes contain hormone-specific cargo modulating trophoblast adhesive capacity: Insights into endometrial-embryo interactions期刊论文

作者: Greening David W Nguyen Hong P.T Elgass Kirstin Simpson Richard J Salamonsen Lois A
DOI:10.1095/biolreprod.115.134890

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页码: 38-38
被引频次: 52
出版者: OXFORD UNIV PRESS INC
期刊名称: Biology of Reproduction
ISSN: 0006-3363
卷期: Volume:94    Issue:2
语言: English
摘要: Embryo implantation into receptive endometrium requires synergistic endometrial-blastocyst interactions within the uterine cavity and is essential for establishing pregnancy. We demonstrate that exosomes (40-150 nm nanovesicles) released from endometrial epithelial cells are an important component of these interactions. We defined the proteome of purified endometrial epithelial-derived exosomes (Exos) influenced by menstrual cycle hormones estrogen (E; proliferative phase) and estrogen plus progesterone (EP; receptive phase) and examined their potential to modify trophoblast function. E-/EP-Exos were uniquely enriched with 254 and 126 proteins, respectively, with 35% newly identified proteins not previously reported in exosome databases. Importantly, EP-Exos protein cargo was related to fundamental changes in implantation: adhesion, migration, invasion, and extracellular matrix remodeling. These findings from hormonally treated ECC1 endometrial cancer cells were validated in human primary uterine epithelial cell-derived exosomes. Functionally, exosomes were internalized by human trophoblast cells and enhanced their adhesive capacity, a response mediated partially through active focal adhesion kinase (FAK) signaling. Thus, exosomes contribute to the endometrialembryo interactions within the human uterine microenvironment essential for successful implantation.
相关主题: Pregnancy, Embryo implantation, Estrogen, Proteomics, Trophoblast, Microenvironment, Progesterone, Exosomes, Endometrium, estrogen, proteomics, CANCER CELLS, pregnancy, progesterone, endometrium, embryo implantation, MASS-SPECTROMETRY, microenvironment, COMPLEMENT REGULATORY PROTEINS, EXTRACELLULAR-MATRIX PROTEINS, REPRODUCTIVE BIOLOGY, exosomes, UTERINE EPITHELIAL-CELLS, DECAY-ACCELERATING FACTOR, HEPARAN-SULFATE PROTEOGLYCAN, IMPLANTATION WINDOW, trophoblast, PROTEOMIC ANALYSIS, NORMAL MENSTRUAL-CYCLE, Exosomes - metabolism, Embryo Implantation - physiology, Trophoblasts - metabolism, Humans, Maternal-Fetal Relations - physiology, Female, Embryo, Mammalian - metabolism, Endometrium - metabolism, Index Medicus,

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