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期刊名称: Biology of Reproduction
Volume:83    Issue:6        Page:909-918
ISSN:0006-3363

Beta-oxidation is essential for mouse oocyte developmental competence and early embryo development期刊论文

作者: Dunning Kylie R Cashman Kara Russell Darryl L Thompson Jeremy G Norman Robert J
DOI:10.1095/biolreprod.110.084145

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页码: 909-918
被引频次: 176
出版者: SOC STUDY REPRODUCTION
期刊名称: Biology of Reproduction
ISSN: 0006-3363
卷期: Volume:83    Issue:6
语言: English
摘要: Oocyte and embryo metabolism are closely linked with their subsequent developmental capacity. Lipids are a potent source of cellular energy, yet little is known about lipid metabolism during oocyte maturation and early embryo development. Generation of ATP from lipids occurs within mitochondria via beta-oxidation of fatty acids, with the rate-limiting step catalyzed by carnitine palmitoyl transferase I (CPT1B), a process also requiring carnitine. We sought to investigate the regulation and role of beta-oxidation during oocyte maturation and preimplantation development. Expression of Cpt1b mRNA, assessed by real-time RT-PCR in murine cumulus-oocyte complexes (COCs), increased following hormonal induction of oocyte maturation and ovulation in vivo with human chorionic gonadotropin (5 IU) and in embryos reaching the blastocyst stage. Beta-oxidation, measured by the production of (H2O)-H-3 from [H-3]palmitic acid, was significantly increased over that in immature COCs following induction of maturation in vitro with epidermal growth factor (3 ng/ml) and follicle-stimulating hormone (50 mlU/ml). The importance of lipid metabolism for oocyte developmental competence and early embryo development was demonstrated by assessing the rate of embryo development following inhibition or upregulation of beta-oxidation with etomoxir (an inhibitor of CPT1B) or L-carnitine, respectively. Inhibition of beta-oxidation during oocyte maturation or zygote cleavage impaired subsequent blastocyst development. In contrast, L-carnitine supplementation during oocyte maturation significantly increased beta-oxidation, improved developmental competence, and in the absence of a carbohydrate energy supply, significantly increased 2-cell cleavage. Thus, carnitine is an important cofactor for developing oocytes, and fatty acids are an important energy source for oocyte and embryo development.
相关主题: Fatty acid oxidation, Beta-oxidation, CPT1B, Embryo development, Oocyte maturation, ATP CONTENT, FOLLICULAR-FLUID, embryo development, CUMULUS CELL COMPLEXES, BOVINE OOCYTES, REPRODUCTIVE BIOLOGY, IN-VITRO MATURATION, beta-oxidation, FATTY-ACID OXIDATION, MITOCHONDRIAL DISTRIBUTION, GENE-EXPRESSION, PREIMPLANTATION EMBRYOS, fatty acid oxidation, oocyte maturation, ENERGY-METABOLISM, Ovulation - metabolism, Carnitine O-Palmitoyltransferase - genetics, Zygote - metabolism, Carnitine - metabolism, Embryo Culture Techniques, RNA, Messenger - metabolism, Granulosa Cells - drug effects, Granulosa Cells - metabolism, Carnitine O-Palmitoyltransferase - antagonists & inhibitors, Cumulus Cells - drug effects, Cumulus Cells - metabolism, Carnitine O-Palmitoyltransferase - metabolism, Mice, Inbred CBA, Oocytes - drug effects, Female, Oogenesis - drug effects, Ovulation - drug effects, Fatty Acids - metabolism, Zygote - cytology, Oocytes - metabolism, Mice, Inbred C57BL, Blastocyst - metabolism, Cells, Cultured, Enzyme Inhibitors - pharmacology, Gene Expression Regulation, Developmental - drug effects, Animals, Zygote - drug effects, Mice, Embryonic Development - drug effects, Index Medicus,

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