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期刊名称: Biology of Reproduction
Volume:37    Issue:5        Page:1283-1289
ISSN:0006-3363

Immunocytochemistry of extracellular matrix in the lamina propria of the rat testis: electron microscopic localization期刊论文

作者: M A Hadley M Dym
DOI:10.1095/biolreprod37.5.1283

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页码: 1283-1289
出版者: Society for the Study of Reproduction
期刊名称: Biology of Reproduction
ISSN: 0006-3363
卷期: Volume:37    Issue:5
语言: English
摘要: The distribution of laminin, type IV collagen, heparan sulfate proteoglycan, and fibronectin was investigated in the rat testicular lamina propria by electron microscopic immunocytochemistry. Distinct patterns were observed for each antigen within the extracellular matrix (ECM) layers of the lamina propria. Laminin, type IV collagen, and heparan sulfate proteoglycan all localized to the seminiferous tubule basement membrane. Type IV collagen and heparan sulfate proteoglycan, but not laminin, localized to the seminiferous tubule side of the peritubular myoid cells. All four of the antigens were localized between the peritubular and lymphatic endothelial cells. Failure to localize fibronectin in the ECM layer between the Sertoli and peritubular myoid cells tends to support the concept that adult Sertoli cells do not produce this protein in vivo. Intracellular immunostaining was insufficient to allow unambiguous identification of the cellular source of any of the ECM molecules.
相关主题: Fibronectins - analysis, Rats, Male, Testis - ultrastructure, Rats, Inbred Strains, Immunoenzyme Techniques, Laminin - analysis, Extracellular Matrix - ultrastructure, Testis - analysis, Animals, Extracellular Matrix - analysis, Heparitin Sulfate - analysis, Collagen - analysis, Index Medicus,

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