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期刊名称: Nature Reviews Genetics
Volume:7    Issue:5        Page:373-384
ISSN:1471-0056

High-throughput RNAi screening in cultured cells: a user's guide期刊论文

作者: Echeverri Christophe J Perrimon Norbert
DOI:10.1038/nrg1836

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页码: 373-384
被引频次: 242
出版者: NATURE PUBLISHING GROUP,Nature Publishing Group
期刊名称: Nature Reviews Genetics
ISSN: 1471-0056
卷期: Volume:7    Issue:5
语言: English
摘要: RNA interference has re-energized the field of functional genomics by enabling genome-scale loss-of-function screens in cultured cells. Looking back on the lessons that have been learned from the first wave of technology developments and applications in this exciting field, we provide both a user's guide for newcomers to the field and a detailed examination of some more complex issues, particularly concerning optimization and quality control, for more advanced users. From a discussion of cell lines, screening paradigms, reagent types and read-out methodologies, we explore in particular the complexities of designing optimal controls and normalization strategies for these challenging but extremely powerful studies.
相关主题: DROSOPHILA-MELANOGASTER CELLS, DOUBLE-STRANDED-RNA, CAENORHABDITIS-ELEGANS, MICRORNA PRECURSORS, JAK/STAT SIGNALING COMPONENTS, MEDIATED ENDOCYTOSIS, GENETICS & HEREDITY, FUNCTIONAL GENOMIC ANALYSIS, SHORT-INTERFERING RNAS, MICROPROCESSOR COMPLEX, MAMMALIAN-CELLS, Gene Transfer Techniques, RNA, Small Interfering - genetics, Automation, Phenotype, Animals, RNA Interference, Models, Biological, Humans, Cells, Cultured, Gene Expression Profiling - methods, Drosophila melanogaster - genetics, Quality Control,

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