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作者 : Senada Nozinovic ;   Anke Reining ;   Yong-Boum Kim ;   Jonas Noeske ;   Kai Schlepckow ;   Jens W?hnert ;   Harald Schwalbe ;  
ISSN : 1547-6286;1555-8584;
出版时间 : 2014
卷期 : 11(5)
起始页码 : 655
终止页码 : 666
摘要 : We report here an in-depth characterization of the aptamer domain of the transcriptional adenine-sensing riboswitch (pbuE) by NMR and fluorescence spectroscopy. By NMR studies, the structure of two aptamer sequences with different lengths of the helix P1, the central element involved in riboswitch conformational switching, was characterized. Hydrogen-bond interactions could be mapped at nucleotide resolution providing information about secondary and tertiary structure, structure homogeneity and dynamics. Our study reveals that the elongation of helix P1 has pronounced effects not only on the local but on the global structure of the apo aptamer domain. The structural differences induced by stabilizing helix P1 were found to be linked to changes of the ligand binding affinity as revealed from analysis of kinetic and thermodynamic data obtained from stopped-flow fluorescence studies. The results provide new insight into the sequence-dependent fine tuning of the structure and function of purine-sensing riboswitches.

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